Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,633,969	C→T	G53D (GGT→GAT)	SAUSA300_RS08080 ←	mannosyl‑glycoprotein endo‑beta‑N‑acetylglucosamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,633,969	0	C	T	100.0%	17.6 / NA	8	G53D (GGT→GAT)	SAUSA300_RS08080	mannosyl‑glycoprotein endo‑beta‑N‑acetylglucosamidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

ACCCTCTAATTTTCCACCTTTAGCATTTTCGTTAAACTTATCAATACCATTTGCCGAAACATTACCGCCACTAACAGTACAACCAGCATCATCTTTACCTTTATTTAAATCAGGCGCTAACAAAAACATTATAATTACTGGAAATATAAAAAAAGCCACCACACATAAAATG  >  NC_007793/1633872‑1634043                                                                                                  |                                                                           aCCCTCTAATTTTCCACCTTTAGCATTTTCATTAAACTTATCAATACCATTTGCCGAAACATTACCGCCACTAACAGTACAACCAGCATCATCTTTATCt                                                                          >  1:2487393/1‑100 (MQ=255)      ctAATTTTCCACCTTTAGCATTTTCATTAAACTTATCAATACCATTTGCCGAAACATTACCGCCACTAACAGTACAACCAGCATCATCTTTATCtttatt                                                                     >  1:5571898/1‑100 (MQ=255)              ccACCTTTAGCATTTTCATTAAACTTATCAATACCATTTGCCGAAACATTACCGCCACTAACAGTACAACCAGCATCATCTTTATCTTTATTAGAATCAg                                                             >  1:5106618/1‑100 (MQ=255)                       gctttttCATTAAACTTATCAATCCCTTTTGCCAAACCATTACCGCCACTAACAGTACAACCAGCATCATCTTTATCTTTATTTGAATCAGGCGCTAACa                                                    <  1:1649338/97‑1 (MQ=255)                       gCATTTTGATAAAACTTAAAAATCCCATTGGCAAAAACTTTACCGCCACTAACAGTACAACCAGCATCATCTTTATCTTTATTTGAATCAGGCGCTAACa                                                    <  1:1281451/100‑1 (MQ=255)                                taaaaCTTAAAAATACCATTTGCAAAAAATTTACCGCCACTAACAGTACAACCAGCATCATCTTTATCTTTATTTGAATCAGGCGCTAACAAAAACATta                                           <  1:1322591/98‑1 (MQ=255)                                                               tACCGCCACTAACAGTACAACCAGCATCATCTTTATCTTTATTTGAATCAGGCGCTAACAAAAACATTACAATTACTGGAAATGTAAAAAAAGCcaccac            >  1:5408127/1‑100 (MQ=255)                                                                         aaCAGTACAACCAGCATCATCTTTATCTTTATTTGAATCAGGCGCTAACAAAAACATTACAATTACTGGAAATGTAAAAAAAGCCACCACACACAAAatg  >  1:1257724/1‑100 (MQ=255)                                                                                                  |                                                                           ACCCTCTAATTTTCCACCTTTAGCATTTTCGTTAAACTTATCAATACCATTTGCCGAAACATTACCGCCACTAACAGTACAACCAGCATCATCTTTACCTTTATTTAAATCAGGCGCTAACAAAAACATTATAATTACTGGAAATATAAAAAAAGCCACCACACATAAAATG  >  NC_007793/1633872‑1634043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑