Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,140	C→T	Q127Q (CAG→CAA)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,140	0	C	T	100.0%	29.3 / NA	12	Q127Q (CAG→CAA)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/3);  total (9/3)

CGTTTGACCCATAAATACGTTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATCTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGACAAACTGAAAAATTCGACATTTTGTTTGTGTAAATCTTCGACA  >  NC_007793/47074‑47230                                                                   |                                                                                           cGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACt                                                           >  1:1696396/1‑100 (MQ=255)      gccccATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCAt                                                      >  1:2329112/3‑100 (MQ=255)      gccccATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGCATTGACTTCCAt                                                      >  1:779033/3‑100 (MQ=255)            tAAATACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGctg                                                <  1:1685390/100‑2 (MQ=255)                tACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGat                                            <  1:1388698/100‑2 (MQ=255)                  cattCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGATaa                                          <  1:4825060/98‑1 (MQ=255)                        cGACAATGTTACTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGaa                                    >  1:579199/1‑100 (MQ=255)                                                    ccaaCTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCt        >  1:2102646/3‑100 (MQ=255)                                                          cGCAAGTATTTGTAACATGAGTTTACCAGAAGAGGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGAcg  >  1:5134558/1‑99 (MQ=255)                                                          cGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGAcg  >  1:1409144/1‑99 (MQ=255)                                                          cGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGAcg  >  1:3943455/1‑99 (MQ=255)                                                          cGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGAcg  >  1:4291413/1‑99 (MQ=255)                                                                   |                                                                                           CGTTTGACCCATAAATACGTTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATCTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGACAAACTGAAAAATTCGACATTTTGTTTGTGTAAATCTTCGACA  >  NC_007793/47074‑47230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑