Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,633,213	G→A	A305V (GCT→GTT)	SAUSA300_RS08080 ←	mannosyl‑glycoprotein endo‑beta‑N‑acetylglucosamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,633,213	0	G	A	88.9%	15.2 / ‑4.6	9	A305V (GCT→GTT)	SAUSA300_RS08080	mannosyl‑glycoprotein endo‑beta‑N‑acetylglucosamidase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (3/5);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAATGTACGTTCATTAACTTTAAAAGCACCTTCCCAATTCATTTCTGTTATTCTAAATGTTTTACCACCATCACTTACACCAATAACAACAGCCACATGACCATAATGCCCATCATGACCGCCTGCACCTTGTTCAGCAATAAATAATGCACCAGGTTCCGGATTTTTATCAACTTTATAACCCTC  >  NC_007793/1633120‑1633307                                                                                              |                                                                                               ttCAATGTACGTTCATTAACTTTAAAAGCAGCTTCCCAATTCATTTCTGTTATTCTAAATGTTTTACCACCATCACTTACACCAATAACAACAACcacag                                                                                          <  1:3014957/100‑2 (MQ=255)        caCGTTCATTAACTTTAAAAGCAGCTTCCCAATTCATTTCTGTTATTCTAAATGTTTTACCACCATCACTTACACCAACAACAACAACCACATGACCATa                                                                                   >  1:4162447/2‑100 (MQ=255)             tCATTAACTTTAAAAGCAGCTTCCCAATTCATTTCTGTTATTCTAAATGTTTTACCACCATCACTTACACCAATAACAACAACCACATGACCATAATGcc                                                                              >  1:4881659/1‑100 (MQ=255)                   aCTTTAAAAGCAGCTTCCCAATTCATTTCTGTTATTCTAAATGTTTTACCACCATCACTTACACCAATAACAACAACCACATGACCATAATGCCcatcac                                                                        <  1:4073869/100‑2 (MQ=255)                          aaGCAGCTTCCCAATTCATTTCTGTTATTCTAAATGTTTTACCACCATCACTTACACCAATAACAACAACCACATGACCATAATGCCCATCATGACCGcc                                                                 <  1:3948724/100‑1 (MQ=255)                              agcTTCCCAATTCATTTCTGTTATTCTAAATGTTTTACCACCATCACTTACACCAATAACAACAACCACATGACCATAATGCCCATCATGACCGCCTgat                                                             <  1:4200365/98‑3 (MQ=255)                                                 gTTATTCTAAATGTTTTACCACCATCACTTACACCAATAACAACAACCACATGACCATAATGCCCATCATGACCGCCTGCACCTTGTTCAGCaataaata                                          >  1:3890416/1‑100 (MQ=255)                                                        tAAATGTTTTACCACCATCACTTACACCAATAACAACAACCACATGACCATAATGCCCATCATGACCGCCTGCACCTTGTTCAGCAATAAATAATGCAcc                                   <  1:5701998/100‑1 (MQ=255)                                                         aaaTGTTTTACCACCATCACTTACACCAATAACAACAGCCACATGACCATAATGCCCATCATGACCGCCTGCACCTTGTTCAGCAATAAATAATGCACCa                                  >  1:2793338/1‑100 (MQ=255)                                                                                         caacaaCCACATGACCATAATGCCCATCATGACCGCCTGCACCTTGTTCAGCAATAAATAATGCACCAGGTTCCGGATTTTTATCAACTTTATAACCCTc  <  1:4278291/100‑1 (MQ=255)                                                                                              |                                                                                               TTCAATGTACGTTCATTAACTTTAAAAGCACCTTCCCAATTCATTTCTGTTATTCTAAATGTTTTACCACCATCACTTACACCAATAACAACAGCCACATGACCATAATGCCCATCATGACCGCCTGCACCTTGTTCAGCAATAAATAATGCACCAGGTTCCGGATTTTTATCAACTTTATAACCCTC  >  NC_007793/1633120‑1633307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑