Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,632,954	C→T	L40L (TTG→TTA)	SAUSA300_RS08075 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,632,954	0	C	T	100.0%	13.7 / NA	7	L40L (TTG→TTA)	SAUSA300_RS08075	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/5);  total (2/5)

TTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAA  >  NC_007793/1632898‑1633026                                                         |                                                                         tttACTAGTTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGTAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAg                               <  1:4640320/100‑1 (MQ=255)  ttACTAGTTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGTAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAgg                              >  1:1398465/1‑100 (MQ=255)    aCTAGTTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGTAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAggga                            >  1:539229/1‑98 (MQ=255)      tagtttatttaACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGTAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGGAta                          <  1:5785000/97‑2 (MQ=255)           tatttaACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGTAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGGATCTGATa                     <  1:53717/100‑1 (MQ=255)                             tATCTCTTAATATACGTTGTTCTTCCTGTAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGGATCTGATAACCAATTAAAACAGcacg   <  1:578432/100‑2 (MQ=255)                              aTCTCTTAATATACGTTGTTCTTCCTGTAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGGATCTGATAACCAATTAAAACAGCACaa  <  1:5132138/100‑1 (MQ=255)                                                         |                                                                         TTTACTAATTTATTTAACTCTCCATTTTTATCTCTTAATATACGTTGTTCTTCCTGCAAATCTTCAATTTGATTTTGTTTCATTTTAACTTGTTGTTTAGGAATCTGATAACCAATTAAAACAGCACAA  >  NC_007793/1632898‑1633026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑