Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,092	G→A	N143N (AAC→AAT)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,092	0	G	A	100.0%	41.1 / NA	16	N143N (AAC→AAT)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/11);  total (5/11)

TACTATCTGGTATTTTGTCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGACCCATAAATACGTTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATCTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGAC  >  NC_007793/47007‑47188                                                                                      |                                                                                                 tACTATCTGGTATTTTGTCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGtt                                                                                    <  1:3173250/100‑1 (MQ=255)    gaTCTGGTATCTTGTCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGttatt                                                                                 <  1:5284328/99‑1 (MQ=255)         ggTATTTTGTCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGtt                                                                            >  1:3096318/1‑100 (MQ=255)         ggTATTTTGTCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGtt                                                                            >  1:3640612/1‑100 (MQ=255)                  tCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTg                                                                   <  1:3309118/100‑1 (MQ=255)                  tCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTg                                                                   <  1:3534025/100‑1 (MQ=255)                  tCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTg                                                                   <  1:4164250/100‑1 (MQ=255)                  tCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTg                                                                   <  1:770177/100‑1 (MQ=255)                  tCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTg                                                                   <  1:789025/100‑1 (MQ=255)                    aTAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTgag                                                                 <  1:5613390/100‑1 (MQ=255)                                 aaaTTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGCCCCATAAATACACTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGtg                                                    <  1:4983763/100‑2 (MQ=255)                                                                    cGTTTGCCCCATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACt                 >  1:1696396/1‑100 (MQ=255)                                                                         gccccATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCAt            >  1:2329112/3‑100 (MQ=255)                                                                         gccccATAAATACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGCATTGACTTCCAt            >  1:779033/3‑100 (MQ=255)                                                                               tAAATACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGctg      <  1:1685390/100‑2 (MQ=255)                                                                                   tACATTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATTTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGat  <  1:1388698/100‑2 (MQ=255)                                                                                      |                                                                                                 TACTATCTGGTATTTTGTCATAACCTAGTGGTAAATTGCCTTGATAATAGCCTTCTTGGGCACGTCTCGTTTGACCCATAAATACGTTCTCGACAATGTTATTACGTTCGAATTCTGAGAAACTCGCAAGTATCTGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGAC  >  NC_007793/47007‑47188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑