Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,632,335	C→A	K31N (AAG→AAT)	SAUSA300_RS08070 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,632,335	0	C	A	86.7%	32.7 / ‑0.9	15	K31N (AAG→AAT)	SAUSA300_RS08070	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (4/9);  total (4/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACATTTTTATACTTCTTTGTATTTTTCACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCAT  >  NC_007793/1632280‑1632427                                                        |                                                                                             cAACATTTTTATACTTCTTTGTATTTTTCACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGc                                                  <  1:4721570/100‑1 (MQ=255)                                 tcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATa                  >  1:2390683/7‑100 (MQ=255)                                 tcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATa                  >  1:2946612/7‑100 (MQ=255)                                  cactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATAc                 >  1:4568314/6‑100 (MQ=255)                                   actcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACa                <  1:2683281/96‑1 (MQ=255)                                    ctcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACaa               >  1:2131754/4‑100 (MQ=255)                                    ctcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACaa               <  1:5736548/97‑1 (MQ=255)                                       aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc            <  1:1147150/100‑1 (MQ=255)                                       aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc            <  1:2204886/100‑1 (MQ=255)                                       aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc            <  1:2720558/100‑1 (MQ=255)                                       aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc            <  1:2887236/100‑1 (MQ=255)                                       aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc            <  1:484466/100‑1 (MQ=255)                                       aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc            <  1:521497/100‑1 (MQ=255)                                        tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa           <  1:4389652/100‑1 (MQ=255)                                                 tttCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCtagt  <  1:2599159/100‑4 (MQ=255)                                                        |                                                                                             CAACATTTTTATACTTCTTTGTATTTTTCACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCAT  >  NC_007793/1632280‑1632427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑