Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,632,043	T→C	intergenic (‑81/+28)	SAUSA300_RS08065 ← / ← SAUSA300_RS08070	integrase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,632,043	0	T	C	100.0%	41.4 / NA	17	intergenic (‑81/+28)	SAUSA300_RS08065/SAUSA300_RS08070	integrase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/17);  total (0/17)

GTTTTTTTCTTTTTTTCATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCATCGCTAAAGCGTATAGGGAGTTGATTCTTTATTTTTTCATATTATTTTCTTCATAAACTGGTTTATTATCTTTCTCAA  >  NC_007793/1631944‑1632120                                                                                                    |                                                                              gTTTTTTTCTTTTTTTCATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCAt                                                                               <  1:5627267/100‑1 (MQ=255) gTTTTTTTCTTTTTTTCATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCAt                                                                               <  1:203092/100‑1 (MQ=255)   ttttttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCg                                                                             <  1:3387569/100‑1 (MQ=255)    tttttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCgt                                                                            <  1:1460001/100‑2 (MQ=255)    tttttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCgt                                                                            <  1:1211083/100‑2 (MQ=255)     ttttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCgtc                                                                           <  1:1928603/100‑3 (MQ=255)     ttttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCt                                                                           <  1:1783116/100‑1 (MQ=255)      tttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTa                                                                          <  1:2753922/100‑1 (MQ=255)      tttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTa                                                                          <  1:5135226/100‑1 (MQ=255)       ttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTaa                                                                         <  1:2353928/100‑1 (MQ=255)       ttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTaa                                                                         <  1:3700644/100‑1 (MQ=255)       ttctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTaa                                                                         <  1:4429483/100‑1 (MQ=255)         ctttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTAAAg                                                                       <  1:2681673/100‑1 (MQ=255)          gttttttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTagcac                                                                      <  1:2307024/99‑5 (MQ=255)              tttcATGTATATCCTCCTTTGCTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTAAAGCGtat                                                                  <  1:2391754/100‑1 (MQ=255)              tttcATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTAAAGCGtat                                                                  <  1:2195018/100‑1 (MQ=255)                                                              gTTATAAGAGGATATATGTTCATCAACTCCCGATACCACCGCTAAAGCGTATAGGGAGTTGATTCTTTATTTTTTCGTATTATTTTCTTCATAAACTGGt                  <  1:4435323/100‑1 (MQ=255)                                                                 ataAGAGGATATATGTTCATCAACTCCCGATACCACCGCTAAAGCGTATAGGGAGTTGATTCTTTATTTTTTCATATTATTTTCTTCATAAACTGGtttg               <  1:2626754/100‑2 (MQ=255)                                                                              tGTTCATCAACTCCCGATACCACCGCTAAAGCGTATAGGGAGTTGATTCTTTATTTTTTCATATTATTTTCTTCATAAACTGGTTtatgattactttgat  <  1:4535924/100‑10 (MQ=255)                                                                                                    |                                                                              GTTTTTTTCTTTTTTTCATGTATATCCTCCTTTACTTTTAAAAAGGTTATTTATAAAATAAGTTATAAGAGGATATATGTTCATCAACTCCCGATACCATCGCTAAAGCGTATAGGGAGTTGATTCTTTATTTTTTCATATTATTTTCTTCATAAACTGGTTTATTATCTTTCTCAA  >  NC_007793/1631944‑1632120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑