Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,631,278	G→T	P6P (CCC→CCA)	SAUSA300_RS08060 ←	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,631,278	0	G	T	96.0%	62.0 / ‑3.7	25	P6P (CCC→CCA)	SAUSA300_RS08060	integrase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (2/22);  total (2/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

ATTCATCTTCTATTTCTTCAAATAGAGCAAACTCCTTACCTCTATCCATAGTTATACTTTTGGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCATATAAATATCTTGTCTT  >  NC_007793/1631217‑1631370                                                              |                                                                                             attcaTCTTCTATTTGTTCAAATAGAGCGAACTCCTTCCCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACa                                                        <  1:1251272/100‑1 (MQ=255)       cttctATTTGTTCAAATAGAGCGAACTCCTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTAc                                                  <  1:119011/100‑1 (MQ=255)        ttctATTTGTTCAAATAGAGCGAACTCCTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTAcg                                                 <  1:5443369/100‑2 (MQ=255)         tctATTTGTTCAAATAGAGCGAACTCCTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACag                                                <  1:4227015/100‑2 (MQ=255)          ctATTTGTTCAAATAGAGCGAACTCCTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTATAAACTACAtc                                               <  1:5680591/100‑2 (MQ=255)          ctATTTCTTCAAATAGAGCAAGCTCCTTACCTCTATCCATAGTTATACTTTTGGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACAtt                                               <  1:175097/100‑1 (MQ=255)               tgttcAAATAGAGCGAACTCCTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGAtt                                          <  1:4513611/98‑1 (MQ=255)                       tAGAGCGAACTCCTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACgtgca                                  <  1:5498284/100‑3 (MQ=255)                         gagCGAACTCCTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTAtt                                <  1:755429/100‑1 (MQ=255)                               aCTCCTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATct                          <  1:468387/100‑1 (MQ=255)                                  tccgatctctATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATctact                       <  1:3418249/94‑1 (MQ=255)                                  ccTTACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATctact                       >  1:2124762/1‑100 (MQ=255)                                     ttccgatctCCATTGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGc                    <  1:101705/92‑1 (MQ=255)                                     tACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAACCTACATTGGATTTACGTGTATTTAATCTACTAGc                    <  1:1850951/100‑1 (MQ=255)                                     tACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGc                    <  1:5272553/100‑1 (MQ=255)                                     tACCTCTATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGc                    <  1:5637289/100‑1 (MQ=255)                                       ccgatctCCATAGTTACGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCat                  <  1:2107646/94‑1 (MQ=255)                                        ctctATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCata                 <  1:2996173/100‑1 (MQ=255)                                         tctATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCatat                <  1:4731479/100‑1 (MQ=255)                                          ctATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACACTGGATTTACGTGTATTTAATCTACTAGCAtata               <  1:1705578/100‑1 (MQ=255)                                          cgaTCTATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCAtata               <  1:5531081/98‑1 (MQ=255)                                           tATCCATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCATATaa              <  1:3815778/100‑1 (MQ=255)                                              ccATAGTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCATATAAata           >  1:1980261/1‑100 (MQ=255)                                                   gTTATGCTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCATATAAATATCTTg      <  1:2124652/100‑1 (MQ=255)                                                       tgcTTTTTGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCATATAAATATCTTGtttt  <  1:2058392/98‑1 (MQ=255)                                                              |                                                                                             ATTCATCTTCTATTTCTTCAAATAGAGCAAACTCCTTACCTCTATCCATAGTTATACTTTTGGGTAATAATGGTTTCATAATATTTATAATCTCATTACAAACTACATTGGATTTACGTGTATTTAATCTACTAGCATATAAATATCTTGTCTT  >  NC_007793/1631217‑1631370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑