Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,949	C→G	C191S (TGC→TCC)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,949	0	C	G	100.0%	44.9 / NA	18	C191S (TGC→TCC)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/18);  total (0/18)

TGAACTAATACTGAAAGGTTTCCCCTTTTTAGTGACATATCCTTTGTGATTCAATGCATTGGCAATTTTACGATAGCCATGTCCTTTGGCATAGCACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTATCTGGT  >  NC_007793/46855‑47017                                                                                               |                                                                     agAACTAATACTAAAAGGTTTCCCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:369472/99‑1 (MQ=255) agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:2520688/99‑1 (MQ=255) agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:936344/99‑1 (MQ=255) agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:5372755/99‑1 (MQ=255) agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:5279726/99‑1 (MQ=255) agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:5207442/99‑1 (MQ=255) agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:265076/99‑1 (MQ=255) agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:1582789/99‑1 (MQ=255) agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTAGTGATTCAATGCATTGGCTAATTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                                 <  1:3720532/99‑1 (MQ=255)  gAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTcat                                                                <  1:480023/100‑3 (MQ=255)    aCTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGagt                                                              <  1:4507590/100‑3 (MQ=255)                                        gccTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTacac                          <  1:5485368/99‑4 (MQ=255)                                           tttGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCAtt                       <  1:3897481/100‑1 (MQ=255)                                                         cATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACt         <  1:4881528/100‑1 (MQ=255)                                                            tGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTAtt      <  1:4216822/100‑2 (MQ=255)                                                             ggCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTATCt     <  1:3304032/100‑1 (MQ=255)                                                                taTTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTATCTGGt  <  1:3291026/99‑1 (MQ=255)                                                                taTTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTATCTGGt  <  1:5417356/99‑1 (MQ=255)                                                                                               |                                                                     TGAACTAATACTGAAAGGTTTCCCCTTTTTAGTGACATATCCTTTGTGATTCAATGCATTGGCAATTTTACGATAGCCATGTCCTTTGGCATAGCACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTATCTGGT  >  NC_007793/46855‑47017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑