Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,630,832	C→T	intergenic (‑245/+110)	SAUSA300_RS08055 ← / ← SAUSA300_RS08060	acetyl‑CoA carboxylase biotin carboxyl carrier protein subunit/integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,630,832	0	C	T	100.0%	18.7 / NA	9	intergenic (‑245/+110)	SAUSA300_RS08055/SAUSA300_RS08060	acetyl‑CoA carboxylase biotin carboxyl carrier protein subunit/integrase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/9);  total (0/9)

ATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTACATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTT  >  NC_007793/1630770‑1630928                                                               |                                                                                                 atatTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGc                                                             <  1:3766588/100‑1 (MQ=255)                              aagaagCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCgtcctgt                                <  1:4646989/100‑1 (MQ=255)                                  agCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAGATTTATTCgtcctgtcctc                            <  1:5098059/100‑2 (MQ=255)                                  agCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCgtcctgtcctg                            <  1:4275433/100‑2 (MQ=255)                                  agCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCtt                            <  1:5094878/100‑1 (MQ=255)                                       tttACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCcgcg                       <  1:4513965/100‑1 (MQ=255)                                          aCTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTc                    <  1:1883407/100‑1 (MQ=255)                                             aGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCaag                 <  1:3224329/100‑3 (MQ=255)                                                            aaaTCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCtt  <  1:4627138/100‑1 (MQ=255)                                                               |                                                                                                 ATATTACTAAAAGAGTACAGAAGAAGAAAAAGAAGCATTTTACTAGTAATATCTGTCAAAAACCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTACATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTT  >  NC_007793/1630770‑1630928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑