Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,628,171	C→T	G54D (GGT→GAT)	SAUSA300_RS08040 ←	N utilization substance protein B homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,628,171	0	C	T	100.0%	263.1 / NA	92	G54D (GGT→GAT)	SAUSA300_RS08040	N utilization substance protein B homolog
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/91);  total (1/91)

TTAAAATAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAA  >  NC_007793/1628082‑1628262                                                                                          |                                                                                            ttAAAATAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATGTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGcc                                                                                   <  1:1874859/100‑1 (MQ=255) ttAAAATAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGcc                                                                                   <  1:1732242/100‑1 (MQ=255) ttAAAATAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGcc                                                                                   <  1:234860/100‑1 (MQ=255)   gtgctcttccGATCTGTTTCTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAg                                                                                 <  1:4242408/91‑1 (MQ=255)   aaaaTAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAg                                                                                 <  1:3870631/100‑1 (MQ=255)   aaaaTAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAg                                                                                 <  1:3536316/100‑1 (MQ=255)    aaaTAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAgc                                                                                <  1:2459238/100‑2 (MQ=255)    aaaTAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGa                                                                                <  1:2415168/100‑1 (MQ=255)     tcttccgatctCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGaa                                                                               <  1:325918/90‑1 (MQ=255)     aataatACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAggt                                                                               <  1:1604097/100‑3 (MQ=255)     aataatACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAgca                                                                               <  1:1612080/100‑3 (MQ=255)     aataatACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGat                                                                               <  1:366264/100‑2 (MQ=255)     aataatACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGaa                                                                               <  1:2553394/100‑1 (MQ=255)     aataatACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGaa                                                                               <  1:4413078/100‑1 (MQ=255)     aataatACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGaa                                                                               <  1:716907/100‑1 (MQ=255)        ttccgatcttgTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACt                                                                            <  1:1775855/90‑1 (MQ=255)        aataCGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACt                                                                            <  1:1958713/100‑1 (MQ=255)        aataCGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACt                                                                            <  1:4345164/100‑1 (MQ=255)           aCGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGc                                                                         <  1:1200076/100‑1 (MQ=255)            gatctcGTATTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGcc                                                                        <  1:4373798/95‑1 (MQ=255)            cGATCTGGTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGcc                                                                        <  1:5609582/100‑1 (MQ=255)            cGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGcc                                                                        <  1:181113/100‑1 (MQ=255)            cGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGcc                                                                        <  1:2745409/100‑1 (MQ=255)            cGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGcc                                                                        <  1:2199087/100‑1 (MQ=255)            cGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGcc                                                                        <  1:4237925/100‑1 (MQ=255)             gATCTGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:346942/100‑1 (MQ=255)             gATCTGTTTTTAATAAACGTGCAATAGTCCAATCTTCTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:5144632/100‑1 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGcat                                                                       <  1:1919704/100‑3 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:5202322/100‑1 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:753056/100‑1 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:4313143/100‑1 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:3373864/100‑1 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:285818/100‑1 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:5490276/100‑1 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:5671484/100‑1 (MQ=255)             gATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:529438/100‑1 (MQ=255)             cgatctgttttAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:2138890/93‑1 (MQ=255)             atctcGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCa                                                                       <  1:103396/96‑1 (MQ=255)              aTCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATGCAGGTTCGTGATCTTTAACGCCAGAAACTAGCCaa                                                                      <  1:3861105/100‑1 (MQ=255)              aTCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCaa                                                                      <  1:5736138/100‑1 (MQ=255)               tCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCaga                                                                     <  1:4802706/100‑3 (MQ=255)               tCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCaag                                                                     <  1:2760385/100‑2 (MQ=255)               tCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCaaa                                                                     <  1:863739/100‑2 (MQ=255)               atctgttttAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAAt                                                                     <  1:4686679/95‑1 (MQ=255)                ccGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCagag                                                                    <  1:2304369/100‑4 (MQ=255)                ccGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCaagt                                                                    <  1:5267023/100‑3 (MQ=255)                ccGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCaaag                                                                    <  1:1564464/100‑3 (MQ=255)                ccGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATg                                                                    <  1:3703542/100‑1 (MQ=255)                ccGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATg                                                                    <  1:3450531/100‑1 (MQ=255)                ccGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATg                                                                    <  1:2233667/100‑1 (MQ=255)                ccGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATg                                                                    <  1:2619660/100‑1 (MQ=255)                ccGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATg                                                                    <  1:3211814/100‑1 (MQ=255)                 cGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGa                                                                   <  1:2333427/100‑1 (MQ=255)                  atttttAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGaa                                                                  <  1:928082/99‑1 (MQ=255)                   tttttAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAAt                                                                 <  1:430356/100‑1 (MQ=255)                          taaACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCg                                                          <  1:4550061/100‑1 (MQ=255)                                tGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCt                                                    <  1:3853204/100‑1 (MQ=255)                                tGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCt                                                    <  1:4963077/100‑1 (MQ=255)                                 gCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTa                                                   <  1:591918/100‑1 (MQ=255)                                        tCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTgg                                            <  1:4128998/100‑1 (MQ=255)                                        tCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTgg                                            <  1:5399673/100‑1 (MQ=255)                                        tCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTgg                                            <  1:5773943/100‑1 (MQ=255)                                        tCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTgg                                            <  1:513002/100‑1 (MQ=255)                                        tCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTgg                                            <  1:5061234/100‑1 (MQ=255)                                        tCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAAGTCGAAGTCTAAATCTgg                                            <  1:5432831/100‑1 (MQ=255)                                         ccAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTggg                                           <  1:1780538/100‑2 (MQ=255)                                          cAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGaa                                          <  1:2772187/100‑2 (MQ=255)                                           aaTCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGAtt                                         <  1:3781491/100‑1 (MQ=255)                                           aaTCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGAta                                         <  1:1811723/100‑2 (MQ=255)                                           aaTCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGAta                                         <  1:1254584/100‑2 (MQ=255)                                           aaTCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGATATTTAACGACAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGAtt                                         <  1:541183/100‑1 (MQ=255)                                           aaTCTGTTAAATAAGGACTAAGTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGAtt                                         <  1:5484931/100‑1 (MQ=255)                                                              aaTTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAGGcaa                      <  1:3176002/100‑3 (MQ=255)                                                              aaTTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGcaa                      <  1:4222333/100‑3 (MQ=255)                                                              aaTTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCtt                      <  1:4687626/100‑1 (MQ=255)                                                              aaTTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCtt                      <  1:1779507/100‑1 (MQ=255)                                                              aaTTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCtt                      <  1:1774971/100‑1 (MQ=255)                                                              aaTTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAAAAAAGCtt                      <  1:5134551/100‑1 (MQ=255)                                                               aTTGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTa                     <  1:1543841/100‑1 (MQ=255)                                                                ttGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTag                    <  1:5793415/100‑2 (MQ=255)                                                                ttGTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCAAAATCTGGATTATCGTCCTTAATAAAGCTTag                    <  1:5174343/100‑2 (MQ=255)                                                                  gTCTCGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTAtac                  <  1:301076/100‑3 (MQ=255)                                                                   tctcGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGc                 <  1:5068223/100‑1 (MQ=255)                                                                     tcGTCTAATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTTGATTATCGTCTTTAATAAAGCTTATCgtca               <  1:3514823/100‑4 (MQ=255)                                                                     gcGTCTAACACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCtg               <  1:240694/99‑2 (MQ=255)                                                                       gTCTGATACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCa             <  1:5259464/100‑1 (MQ=255)                                                                           taatcAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTa         <  1:1865923/96‑1 (MQ=255)                                                                           aaTACAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTa         >  1:4912350/1‑100 (MQ=255)                                                                               cAGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGt     <  1:5122336/100‑1 (MQ=255)                                                                                aGGTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGtt    <  1:4459919/100‑1 (MQ=255)                                                                                  gTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTaa  <  1:275803/100‑1 (MQ=255)                                                                                  gTTCGTGATCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTaa  <  1:3007246/100‑1 (MQ=255)                                                                                          |                                                                                            TTAAAATAATACGATCCGTTTTTAATAAACGTGCAATAGTCCAATCTTTTAAATAAGGACTAATTGTCTCGTCTAATACAGGTTCGTGACCTTTAACGCCAGAAACTAGCCAATGAATAAATTCGAAGTCTAAATCTGGATTATCGTCTTTAATAAAGCTTATCGCTTCATTTATCGTTAA  >  NC_007793/1628082‑1628262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑