Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,605,121	T→A	M96K (ATG→AAG)	SAUSA300_RS07930 →	pyrroline‑5‑carboxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,605,121	0	T	A	100.0%	181.0 / NA	65	M96K (ATG→AAG)	SAUSA300_RS07930	pyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (62/3);  total (62/3)

TGTATTTTTAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTATGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCAAGTTGGA  >  NC_007793/1605029‑1605212                                                                                             |                                                                                            tGTATTTTTAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGt                                                                                      <  1:2630906/100‑1 (MQ=255)        ttAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCga                                                                               >  1:552303/1‑100 (MQ=255)        atAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATCAAGGCAgatcggaaga                                                                               >  1:1161346/2‑91 (MQ=255)         tAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCgat                                                                              >  1:3922975/1‑100 (MQ=255)         tAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCgat                                                                              >  1:2828685/1‑100 (MQ=255)         tAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCgat                                                                              >  1:1520970/1‑100 (MQ=255)         tAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCaat                                                                              >  1:2351932/1‑100 (MQ=255)           cgTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCgattg                                                                            >  1:2787699/2‑100 (MQ=255)              aCCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCgattgatt                                                                         >  1:3518551/1‑100 (MQ=255)              aCCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCgattgatt                                                                         >  1:2221016/1‑100 (MQ=255)               ccAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATta                                                                        >  1:3305732/1‑100 (MQ=255)               ccAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATta                                                                        >  1:2689184/1‑100 (MQ=255)                cAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATtat                                                                       >  1:973189/1‑100 (MQ=255)                 aaaCCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATtata                                                                      >  1:5182379/1‑100 (MQ=255)                   gccACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATAtt                                                                    >  1:3754119/2‑100 (MQ=255)                   aCCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATAtt                                                                    >  1:761027/1‑100 (MQ=255)                    ccACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTa                                                                   >  1:5076430/1‑100 (MQ=255)                    ccACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTa                                                                   >  1:5576851/1‑100 (MQ=255)                     cacaTGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTaa                                                                  <  1:919993/100‑1 (MQ=255)                                 aTGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGa                                                      >  1:5099186/1‑100 (MQ=255)                                      ctAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGcc                                                 >  1:4747156/1‑100 (MQ=255)                                      ctAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGcc                                                 >  1:422966/1‑100 (MQ=255)                                      ctAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGcc                                                 >  1:4428399/1‑100 (MQ=255)                                       caGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCa                                                >  1:222322/2‑100 (MQ=255)                                        aGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCaa                                               >  1:2861905/1‑100 (MQ=255)                                        aGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCaa                                               >  1:4612026/1‑100 (MQ=255)                                        aGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCaa                                               >  1:4799950/1‑100 (MQ=255)                                         gCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCaaa                                              >  1:1176939/1‑100 (MQ=255)                                         gCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCaaa                                              >  1:4964441/1‑100 (MQ=255)                                         gCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCaaa                                              >  1:1730813/1‑100 (MQ=255)                                          cAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCaaaa                                             >  1:38909/1‑100 (MQ=255)                                           aaCACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGTAGGTATTCCGTTTGATTATATTAAACAACAATTAGAATGCCAAAAt                                            >  1:3403385/1‑100 (MQ=255)                                           aaCACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAAt                                            >  1:549040/1‑100 (MQ=255)                                            ccacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAATGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATc                                           >  1:72886/2‑100 (MQ=255)                                            ccacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATc                                           >  1:3484863/2‑100 (MQ=255)                                            ccacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATc                                           >  1:3752990/2‑100 (MQ=255)                                            ccacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATc                                           >  1:5273275/2‑100 (MQ=255)                                            acacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATc                                           >  1:1376034/1‑100 (MQ=255)                                            acacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATc                                           >  1:2034596/1‑100 (MQ=255)                                             cacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGTTTGATTATATTAAACAACAATTAGAATGCCAAAATcc                                          >  1:5414637/1‑100 (MQ=255)                                             cacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATcc                                          >  1:508508/1‑100 (MQ=255)                                             cacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATcc                                          >  1:4009401/1‑100 (MQ=255)                                             cacGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATcc                                          >  1:4600649/1‑100 (MQ=255)                                               cGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAg                                        >  1:1119030/1‑100 (MQ=255)                                               cGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAg                                        >  1:5275250/1‑100 (MQ=255)                                                    cAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCt                                   <  1:3739576/100‑1 (MQ=255)                                                     taaCCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTa                                  >  1:1336069/2‑100 (MQ=255)                                                     aaaCCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTa                                  >  1:1829277/1‑100 (MQ=255)                                                      caCCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAg                                 >  1:2820926/2‑100 (MQ=255)                                                      caCCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAg                                 >  1:3423407/2‑100 (MQ=255)                                                      aaCCACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAg                                 >  1:2210263/1‑100 (MQ=255)                                                       cccACATATTACAAAAGACAATTGCTTCGTTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGa                                >  1:1074387/2‑100 (MQ=255)                                                       cccACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGa                                >  1:1194859/2‑100 (MQ=255)                                                       aCCACATATTACAAAAGACAATTGCTTCACTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGa                                >  1:4925427/1‑100 (MQ=255)                                                        ccACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGaa                               >  1:4332102/1‑100 (MQ=255)                                                        ccACATATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGaa                               >  1:1525094/1‑100 (MQ=255)                                                         cacaTATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAAt                              >  1:124815/1‑100 (MQ=255)                                                          acaTATTACAAAAGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAAtt                             >  1:3828819/1‑100 (MQ=255)                                                                     aaGACAATTGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAacac                  >  1:693866/1‑100 (MQ=255)                                                                             tGCTTCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAACACAAATgcgc          >  1:1246394/1‑100 (MQ=255)                                                                               cctCATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCaa        >  1:3353690/3‑100 (MQ=255)                                                                                  gccctCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCAAGtt     >  1:3698122/5‑100 (MQ=255)                                                                                  cATTTCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCAAGtt     >  1:5473938/1‑100 (MQ=255)                                                                                     ttCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCAAGTTGGa  >  1:1059110/1‑100 (MQ=255)                                                                                     ttCAATTAAGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCAAGTTGGa  >  1:464848/1‑100 (MQ=255)                                                                                             |                                                                                            TGTATTTTTAGGTACCAAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAATTGCTTCATTTCAATTATGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGCCAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCAAGTTGGA  >  NC_007793/1605029‑1605212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑