Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,918	A→T	I201I (ATT→ATA)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,918	0	A	T	100.0%	120.4 / NA	44	I201I (ATT→ATA)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/42);  total (2/42)

TATAGAAAGGGTTAGCTAAGATGTATGTGATTGAACTAATACTGAAAGGTTTCCCCTTTTTAGTGACATATCCTTTGTGATTCAATGCATTGGCAATTTTACGATAGCCATGTCCTTTGGCATAGCACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTATCT  >  NC_007793/46824‑47014                                                                                               |                                                                                                 tataGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTAtttt                                                                                             >  1:3461329/1‑100 (MQ=255)    aGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACg                                                                                          >  1:1110348/1‑100 (MQ=255)                 tAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCt                                                                             <  1:2606793/100‑1 (MQ=255)                   atatatatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCttt                                                                           <  1:3008391/95‑1 (MQ=255)                      tatatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGc                                                                        <  1:610561/98‑1 (MQ=255)                      tatatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGc                                                                        <  1:1258180/98‑1 (MQ=255)                      tatatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGc                                                                        <  1:4482214/98‑1 (MQ=255)                      tatatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGc                                                                        <  1:2030079/98‑1 (MQ=255)                       ataggtgATAGAACTATTACT‑AAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:1092827/96‑1 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCag                                                                      <  1:5199615/100‑2 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:5045592/100‑1 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:3583442/100‑1 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:2753798/100‑1 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:538591/100‑1 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:1776020/100‑1 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:538974/100‑1 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:5660355/100‑1 (MQ=255)                        tatgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCCTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:4797325/100‑1 (MQ=255)                        tatgtGATAAAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAt                                                                      <  1:4312406/100‑1 (MQ=255)                         atgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCagt                                                                     <  1:1869065/100‑3 (MQ=255)                         atgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAtt                                                                     <  1:2955066/100‑2 (MQ=255)                         atgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCAtt                                                                     <  1:3306724/100‑2 (MQ=255)                         atgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATa                                                                     <  1:3764844/100‑1 (MQ=255)                         atgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATa                                                                     <  1:4025662/100‑1 (MQ=255)                         atgtGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATa                                                                     <  1:157734/100‑1 (MQ=255)                           gtgATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAgg                                                                   <  1:1124748/100‑2 (MQ=255)                           gtgATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAgg                                                                   <  1:5666495/100‑2 (MQ=255)                           gtgATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAgg                                                                   <  1:2551880/100‑2 (MQ=255)                                agAACTAATACTAAAAGGTTTCCCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:369472/99‑1 (MQ=255)                                agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:5207442/99‑1 (MQ=255)                                agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:936344/99‑1 (MQ=255)                                agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:1582789/99‑1 (MQ=255)                                agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:2520688/99‑1 (MQ=255)                                agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:5372755/99‑1 (MQ=255)                                agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:5279726/99‑1 (MQ=255)                                agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:265076/99‑1 (MQ=255)                                agAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTAGTGATTCAATGCATTGGCTAATTTACGATAGCCATGTCCTTTGGCATAGGACTCg                                                              <  1:3720532/99‑1 (MQ=255)                                 gAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTcat                                                             <  1:480023/100‑3 (MQ=255)                                   aCTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGagt                                                           <  1:4507590/100‑3 (MQ=255)                                                                       gccTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTacac                       <  1:5485368/99‑4 (MQ=255)                                                                          tttGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCAtt                    <  1:3897481/100‑1 (MQ=255)                                                                                        cATTGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACt      <  1:4881528/100‑1 (MQ=255)                                                                                           tGGCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTAtt   <  1:4216822/100‑2 (MQ=255)                                                                                            ggCTATTTTACGATAGCCATGTCCTTTGGCATAGGACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTATCt  <  1:3304032/100‑1 (MQ=255)                                                                                               |                                                                                                 TATAGAAAGGGTTAGCTAAGATGTATGTGATTGAACTAATACTGAAAGGTTTCCCCTTTTTAGTGACATATCCTTTGTGATTCAATGCATTGGCAATTTTACGATAGCCATGTCCTTTGGCATAGCACTCGAATATATATTTTACAATATTAGCTTCATGTTGGTTAATCATTAGCTCGTGTTTACTATCT  >  NC_007793/46824‑47014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑