Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,586,043	C→G	G22A (GGA→GCA)	SAUSA300_RS07790 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,586,043	0	C	G	100.0%	29.7 / NA	14	G22A (GGA→GCA)	SAUSA300_RS07790	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (1/13);  total (1/13)

AATCTCCGTCGATAACTCCTATTGATGAGAAAAGCAAAATATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTCCGCTTTTTTCAGATAATAAGTCTTTTACTTCTTCCTGAGTCATGTCTTTATATTTTTTAGTCATTGTTGACTTCCTCCTCGTTT  >  NC_007793/1585954‑1586127                                                                                          |                                                                                     aatCTCCGTCGATAACTCCTATTGATGAGAAAAGCAAAATATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTc                                                                            <  1:1968931/100‑1 (MQ=255) aatCTCCGTCGATAACTCCTATTGATGAGAAAAGCAAAATATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTc                                                                            <  1:4658337/100‑1 (MQ=255)                              aaaaGCAAAATATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTCCAGATAATAAGTCTTTTATTTCTTCCTgct                                               <  1:4812023/100‑3 (MQ=255)                               aaaGCAAAATATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGt                                              <  1:5413007/100‑1 (MQ=255)                                 aGCAAAATATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGtgt                                            <  1:2897194/100‑3 (MQ=255)                                   cAAAATATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATg                                          <  1:1888957/100‑1 (MQ=255)                                    aaaaTATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATGt                                         <  1:1343670/100‑1 (MQ=255)                                    aaaaTATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATGt                                         <  1:4747142/100‑1 (MQ=255)                                       atatCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATGTCtt                                      <  1:3536375/100‑1 (MQ=255)                                        tatCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATGTCttt                                     <  1:1153941/100‑1 (MQ=255)                                                         cccTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATGTCTTTATAATTTTTAGTCAtgg                    >  1:1552225/1‑98 (MQ=25)                                                          ccTTAATTTCTTTCGCTAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATGTCTTTATAATTTTTAGTCAtgga                   <  1:2831839/100‑4 (MQ=25)                                                                          tAATTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATGTCTTTATAATTTTTAGTCATGGTTGACTtcctccgagag   <  1:2200744/100‑6 (MQ=18)                                                                           aaTTCATACAATTCTGCCGTTTTTTCAGATAATAAGTCTTTTATTTCTTCCTGCGTCATGTCTTTATAATTTTTAGTCATGGTTGACTtcctccgttttt  <  1:447537/100‑7 (MQ=18)                                                                                          |                                                                                     AATCTCCGTCGATAACTCCTATTGATGAGAAAAGCAAAATATCAAATTTACTTTCTCCCTTAATTTCTTTCGCTAATTCATACAATTCTCCGCTTTTTTCAGATAATAAGTCTTTTACTTCTTCCTGAGTCATGTCTTTATATTTTTTAGTCATTGTTGACTTCCTCCTCGTTT  >  NC_007793/1585954‑1586127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑