Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,585,833	T→A	H92L (CAC→CTC)	SAUSA300_RS07790 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,585,833	0	T	A	100.0%	43.0 / NA	16	H92L (CAC→CTC)	SAUSA300_RS07790	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/11);  total (5/11)

CTCACCAAATTTGTAATACATTGTTTTAGCCCTCCTTGTTATCATCAATACCGTGAAATTTTTGTGATTTACACATTTGGAGAACATTGACAATGTCTTTATAACTCTTAGTGCTATCCAATAAGGAAGCAAGATCGAAAGTATGACCA  >  NC_007793/1585780‑1585928                                                      |                                                                                                gtcacccgttttGTAATACATTGTTTTAGTCCTCCTTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCttt                                                   >  1:3259601/10‑100 (MQ=18)                   cATTGTTTTAGTCCTCCTTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATc                                 >  1:2214343/1‑100 (MQ=255)                                gatcttggcgtcatctATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTAGCCAATAAGTAAGCa                    <  1:4391051/90‑1 (MQ=255)                                gatctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa                    <  1:2865473/97‑1 (MQ=255)                                gatctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa                    <  1:4950536/97‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa                    <  1:2790828/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa                    <  1:3312560/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa                    <  1:415331/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa                    <  1:5462929/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTAAAAAGGGGGAGAACATTGACAATCTCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa                    <  1:228697/100‑1 (MQ=255)                                 atctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCaa                   <  1:641682/98‑1 (MQ=255)                                           catctATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCAAGATCAAAAGt         <  1:1905053/100‑1 (MQ=255)                                              ctaTACCGAGAATTTTTTGTGATGTACACATTTGGAGAACATTGACAATATCTTTATAACGCTTAGTGCTATCCAATAAGTAAGCAAGATCAAAAGTATg      <  1:5793291/98‑1 (MQ=255)                                                aTACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCAAGATCGGAAgagcaca    >  1:5532923/1‑94 (MQ=255)                                                aTACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGCAGGCAAGATCGGAAgagcaca    >  1:4723013/1‑94 (MQ=255)                                                  aCCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCAAGATCAAAAGTATGACCa  >  1:4581521/1‑100 (MQ=255)                                                      |                                                                                                CTCACCAAATTTGTAATACATTGTTTTAGCCCTCCTTGTTATCATCAATACCGTGAAATTTTTGTGATTTACACATTTGGAGAACATTGACAATGTCTTTATAACTCTTAGTGCTATCCAATAAGGAAGCAAGATCGAAAGTATGACCA  >  NC_007793/1585780‑1585928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑