Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,585,826	A→T	I94I (ATT→ATA)	SAUSA300_RS07790 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,585,826	0	A	T	100.0%	21.9 / NA	10	I94I (ATT→ATA)	SAUSA300_RS07790	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/8);  total (2/8)

CTCACCAAATTTGTAATACATTGTTTTAGCCCTCCTTGTTATCATCAATACCGTGAAATTTTTGTGATTTACACATTTGGAGAACATTGACAATGTCTTTATAACTCTTAGTGCTATCCAATAAGGAAGCAAGATCGAAAGT  >  NC_007793/1585780‑1585921                                               |                                                                                                gtcacccgttttGTAATACATTGTTTTAGTCCTCCTTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCttt                                            >  1:3259601/10‑100 (MQ=18)                   cATTGTTTTAGTCCTCCTTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATc                          >  1:2214343/1‑100 (MQ=255)                                gatcttggcgtcatctATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTAGCCAATAAGTAAGCa             <  1:4391051/90‑1 (MQ=255)                                gatctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa             <  1:2865473/97‑1 (MQ=255)                                gatctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa             <  1:4950536/97‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa             <  1:2790828/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa             <  1:3312560/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa             <  1:415331/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa             <  1:5462929/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTAAAAAGGGGGAGAACATTGACAATCTCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa             <  1:228697/100‑1 (MQ=255)                                 atctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCaa            <  1:641682/98‑1 (MQ=255)                                           catctATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCAAGATCAAAAGt  <  1:1905053/100‑1 (MQ=255)                                               |                                                                                                CTCACCAAATTTGTAATACATTGTTTTAGCCCTCCTTGTTATCATCAATACCGTGAAATTTTTGTGATTTACACATTTGGAGAACATTGACAATGTCTTTATAACTCTTAGTGCTATCCAATAAGGAAGCAAGATCGAAAGT  >  NC_007793/1585780‑1585921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑