Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,828	T→A	E80D (GAA→GAT)	SAUSA300_RS07735 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,828	0	T	A	100.0%	12.2 / NA	6	E80D (GAA→GAT)	SAUSA300_RS07735	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (1/5);  total (1/5)

CGTAAGTTCAGCGTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCAATTGTTAATGTATTAGTCATTTTCCTGATCCTCCTCATATTT  >  NC_007793/1579738‑1579848                                                                                           |                     cctAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGTGTTATTCATTATcgttgtgt             <  1:485701/98‑8 (MQ=255) cctAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGTGTTATTCATTATcgttgtcc             >  1:1415375/3‑93 (MQ=255)      atttagtgTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACCCTTGTAACTGATCGATTGTTAATGTGTTATTCATTATCGTTGtcctcccg        <  1:5190440/93‑3 (MQ=255)        ttaGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGTGTTATTCATTATCGTTGtcctcccatg      <  1:194809/98‑5 (MQ=255)            cGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGTGTTATTCATTATCGTTGtcctcccattgcag  <  1:1789366/100‑9 (MQ=255)            cGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGTGTTATTCATTATCGTTGtcctcccattgcaa  <  1:3289268/100‑9 (MQ=255)                                                                                           |                     CGTAAGTTCAGCGTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCAATTGTTAATGTATTAGTCATTTTCCTGATCCTCCTCATATTT  >  NC_007793/1579738‑1579848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑