Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,746	C→T	L27L (CTG→CTA)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,746	0	C	T	100.0%	41.9 / NA	16	L27L (CTG→CTA)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/14);  total (2/14)

TTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCTATTTTGCTATCTCGTAAGTTCAGCGTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCAATTGTTAATGTATTAGTCATTTTCCTGATCC  >  NC_007793/1579666‑1579837                                                                                 |                                                                                            ttcCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgt                                                                          <  1:5363549/100‑1 (MQ=255)   ccAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgtcg                                                                        >  1:2129334/1‑100 (MQ=255)   ccAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgtcg                                                                        <  1:3851165/100‑1 (MQ=255)                 tttCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtat                                                          <  1:3896818/100‑1 (MQ=255)                   tCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtctgtattt                                                        <  1:442589/100‑1 (MQ=255)                     cAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgt                                                      <  1:3363503/100‑1 (MQ=255)                     cAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgt                                                      <  1:3997210/100‑1 (MQ=255)                      aaTGTATTAAACCATTCAAAGAATTCAACTACATATGAAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgta                                                     <  1:3076615/100‑1 (MQ=255)                                 ccATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATACTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGt                                          <  1:475983/100‑1 (MQ=255)                                  cATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTa                                         <  1:1816431/100‑1 (MQ=255)                                    ttCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAAc                                       <  1:3362928/100‑1 (MQ=255)                                      cAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTg                                     <  1:4751227/100‑1 (MQ=255)                                          gAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATcg                                 <  1:1613239/100‑2 (MQ=255)                                                        atGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGtgtc                   <  1:2170858/100‑4 (MQ=255)                                                                         cctAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGTGTTATTCATTATcgttgtgt  <  1:485701/98‑8 (MQ=255)                                                                         cctAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGTGTTATTCATTATcgttgtcc  >  1:1415375/3‑93 (MQ=255)                                                                                 |                                                                                            TTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCTATTTTGCTATCTCGTAAGTTCAGCGTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCAATTGTTAATGTATTAGTCATTTTCCTGATCC  >  NC_007793/1579666‑1579837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑