Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,739	G→C	R30G (CGA→GGA)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,739	0	G	C	100.0%	43.5 / NA	17	R30G (CGA→GGA)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (2/15);  total (2/15)

CGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCTATTTTGCTATCTCGTAAGTTCAGCGTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCAATTGTTAATGTATT  >  NC_007793/1579647‑1579820                                                                                             |                                                                                  cGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGttt                                                                            >  1:1705626/1‑99 (MQ=255) cGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGttt                                                                            <  1:5449474/100‑2 (MQ=255) cGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGttt                                                                            <  1:178203/100‑2 (MQ=255)                    ttcCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgt                                                         <  1:5363549/100‑1 (MQ=255)                      ccAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgtcg                                                       >  1:2129334/1‑100 (MQ=255)                      ccAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgtcg                                                       <  1:3851165/100‑1 (MQ=255)                                    tttCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtat                                         <  1:3896818/100‑1 (MQ=255)                                      tCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtctgtattt                                       <  1:442589/100‑1 (MQ=255)                                        cAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgt                                     <  1:3363503/100‑1 (MQ=255)                                        cAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgt                                     <  1:3997210/100‑1 (MQ=255)                                         aaTGTATTAAACCATTCAAAGAATTCAACTACATATGAAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgta                                    <  1:3076615/100‑1 (MQ=255)                                                    ccATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATACTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGt                         <  1:475983/100‑1 (MQ=255)                                                     cATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTa                        <  1:1816431/100‑1 (MQ=255)                                                       ttCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAAc                      <  1:3362928/100‑1 (MQ=255)                                                         cAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTg                    <  1:4751227/100‑1 (MQ=255)                                                             gAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATcg                <  1:1613239/100‑2 (MQ=255)                                                                           atGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGtgtc  <  1:2170858/100‑4 (MQ=255)                                                                                             |                                                                                  CGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCTATTTTGCTATCTCGTAAGTTCAGCGTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCAATTGTTAATGTATT  >  NC_007793/1579647‑1579820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑