Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,725	T→A	I34I (ATA→ATT)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,725	0	T	A	100.0%	48.9 / NA	18	I34I (ATA→ATT)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (2/16);  total (2/16)

CTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCTATTTTGCTATCTCGTAAGTTCAGCGTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCAATTGTTAATGTATT  >  NC_007793/1579643‑1579820                                                                                   |                                                                                                cTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTAtctcctgg                                                                                <  1:619525/100‑5 (MQ=255)     cGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGttt                                                                            >  1:1705626/1‑99 (MQ=255)     cGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGttt                                                                            <  1:5449474/100‑2 (MQ=255)     cGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGttt                                                                            <  1:178203/100‑2 (MQ=255)                        ttcCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgt                                                         <  1:5363549/100‑1 (MQ=255)                          ccAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgtcg                                                       >  1:2129334/1‑100 (MQ=255)                          ccAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTAtcgtcg                                                       <  1:3851165/100‑1 (MQ=255)                                        tttCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtat                                         <  1:3896818/100‑1 (MQ=255)                                          tCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtctgtattt                                       <  1:442589/100‑1 (MQ=255)                                            cAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgt                                     <  1:3363503/100‑1 (MQ=255)                                            cAATGTATTAAACCATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgt                                     <  1:3997210/100‑1 (MQ=255)                                             aaTGTATTAAACCATTCAAAGAATTCAACTACATATGAAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTtttgtatttgta                                    <  1:3076615/100‑1 (MQ=255)                                                        ccATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATACTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGt                         <  1:475983/100‑1 (MQ=255)                                                         cATTCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTa                        <  1:1816431/100‑1 (MQ=255)                                                           ttCAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAAc                      <  1:3362928/100‑1 (MQ=255)                                                             cAAAGAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTg                    <  1:4751227/100‑1 (MQ=255)                                                                 gAATTCAACTACATATGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATcg                <  1:1613239/100‑2 (MQ=255)                                                                               atGCAATTTTGCTATCTCCTAAGTTTAGCGTCGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCGATTGTTAATGtgtc  <  1:2170858/100‑4 (MQ=255)                                                                                   |                                                                                                CTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAATTCAACTACATATGCTATTTTGCTATCTCGTAAGTTCAGCGTTGGTATTCTATCGTCGAACTCCTTTTGTATTTGTAATAACTCTTGTAACTGATCAATTGTTAATGTATT  >  NC_007793/1579643‑1579820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑