| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,579,617 | G→A | D70D (GAC→GAT) | SAUSA300_RS07730 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,579,617 | 0 | G | A | 92.9% | 28.5 / ‑4.5 | 14 | D70D (GAC→GAT) | SAUSA300_RS07730 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (2/11); total (2/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-01 | |||||||||||
TCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCCGCTAATTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAA > NC_007793/1579525‑1579709 | tctgtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGcca < 1:3202019/96‑3 (MQ=255)tctgtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGcca < 1:4716030/96‑3 (MQ=255) gtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATccaccaact > 1:4207578/2‑93 (MQ=255) tttAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCt < 1:4850127/100‑1 (MQ=255) cttcttTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCtt < 1:4410432/100‑1 (MQ=255) cttcttTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCtt < 1:4985131/100‑1 (MQ=255) agacgtgtgctctTCCGATCTCACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCttg < 1:2953700/91‑2 (MQ=255) cgaTCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCtag < 1:3168907/98‑3 (MQ=255) gaTCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCAGCTAATTCATCAAGTTGTACGTCTAACGGCTTACCTGGt < 1:5005589/99‑1 (MQ=255) ttccgatcttgaCACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCTGGTttc < 1:4686840/91‑1 (MQ=255) acTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCt < 1:2263180/100‑1 (MQ=255) tCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTa < 1:2102296/100‑1 (MQ=255) aaCGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCaa < 1:674031/100‑1 (MQ=255) gcAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAAAGTTTCCAATGTATTAAACCATTCAAAGaa > 1:5587756/2‑100 (MQ=255) | TCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCCGCTAATTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATTAAACCATTCAAAGAA > NC_007793/1579525‑1579709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |