Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,602	T→A	G75G (GGA→GGT)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,602	0	T	A	91.7%	26.4 / ‑3.6	12	G75G (GGA→GGT)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (1/10);  total (1/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

TCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCCGCTAATTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTA  >  NC_007793/1579525‑1579677                                                                              |                                                                            tctgtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGcca                                                       <  1:3202019/96‑3 (MQ=255) tctgtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGcca                                                       <  1:4716030/96‑3 (MQ=255)    gtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATccaccaact                                                    >  1:4207578/2‑93 (MQ=255)         tttAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCt                                               <  1:4850127/100‑1 (MQ=255)                             cttcttTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCtt                           <  1:4410432/100‑1 (MQ=255)                             cttcttTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCtt                           <  1:4985131/100‑1 (MQ=255)                              agacgtgtgctctTCCGATCTCACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCttg                          <  1:2953700/91‑2 (MQ=255)                                   cgaTCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCtag                     <  1:3168907/98‑3 (MQ=255)                                    gaTCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCAGCTAATTCATCAAGTTGTACGTCTAACGGCTTACCTGGt                    <  1:5005589/99‑1 (MQ=255)                                       ttccgatcttgaCACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCTGGTttc                 <  1:4686840/91‑1 (MQ=255)                                                    acTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCt    <  1:2263180/100‑1 (MQ=255)                                                      tCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGCCAACTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTa  <  1:2102296/100‑1 (MQ=255)                                                                              |                                                                            TCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCCGCTAATTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTA  >  NC_007793/1579525‑1579677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑