Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,532	T→C	N99D (AAT→GAT)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,532	0	T	C	100.0%	30.1 / NA	13	N99D (AAT→GAT)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/5);  total (8/5)

TATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCCGCTAATT  >  NC_007793/1579455‑1579627                                                                              |                                                                                                tatCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGct                                                                           <  1:2706254/100‑1 (MQ=255)     tgtgGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGcttctt                                                                       >  1:2658032/1‑100 (MQ=255)      gtggtgTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCttt                                                                      >  1:919418/1‑100 (MQ=255)                aacaacTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCtcttct                                                            >  1:1479624/1‑100 (MQ=255)                aacaacTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCtcttct                                                            >  1:409353/1‑100 (MQ=255)                    cttGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtgatg                                                        >  1:140410/3‑100 (MQ=255)                               aGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACt                                             <  1:474830/100‑1 (MQ=255)                                  aaaTTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACttac                                          <  1:4304356/100‑3 (MQ=255)                                          tatctttaAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAAt                                  >  1:4160256/1‑100 (MQ=255)                                               ttaAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCa                             >  1:5081596/1‑100 (MQ=255)                                                                       tctgtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGcca     <  1:3202019/96‑3 (MQ=255)                                                                       tctgtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATCCGcca     <  1:4716030/96‑3 (MQ=255)                                                                          gtATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAAACCAAACGCCAACATATccaccaact  >  1:4207578/2‑93 (MQ=255)                                                                              |                                                                                                TATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCCGCTAATT  >  NC_007793/1579455‑1579627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑