Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,515	G→T	N104K (AAC→AAA)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,515	0	G	T	100.0%	38.0 / NA	15	N104K (AAC→AAA)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/3);  total (12/3)

ATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCA  >  NC_007793/1579420‑1579600                                                                                                |                                                                                      aTTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACAttttgt                                                                                   >  1:345257/1‑100 (MQ=255)   tGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGa                                                                                 >  1:2331530/1‑100 (MQ=255)   tGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGa                                                                                 >  1:5145958/1‑100 (MQ=255)    tccAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGaa                                                                                >  1:1428089/2‑100 (MQ=255)    gCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGaa                                                                                >  1:3859205/1‑100 (MQ=255)    gCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGaa                                                                                >  1:5329101/1‑100 (MQ=255)                                    tatCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGct                                                <  1:2706254/100‑1 (MQ=255)                                        tgtgGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGcttctt                                            >  1:2658032/1‑100 (MQ=255)                                         gtggtgTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCttt                                           >  1:919418/1‑100 (MQ=255)                                                   aacaacTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCtcttct                                 >  1:1479624/1‑100 (MQ=255)                                                   aacaacTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCtcttct                                 >  1:409353/1‑100 (MQ=255)                                                       cttGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtgatg                             >  1:140410/3‑100 (MQ=255)                                                                  aGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACt                  <  1:474830/100‑1 (MQ=255)                                                                     aaaTTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACttac               <  1:4304356/100‑3 (MQ=255)                                                                             tatctttaAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAAt       >  1:4160256/1‑100 (MQ=255)                                                                                  ttaAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCa  >  1:5081596/1‑100 (MQ=255)                                                                                                |                                                                                      ATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCA  >  NC_007793/1579420‑1579600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑