Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,480	T→C	D116G (GAC→GGC)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,480	0	T	C	100.0%	44.3 / NA	18	D116G (GAC→GGC)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/6);  total (12/6)

GAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATG  >  NC_007793/1579384‑1579573                                                                                                 |                                                                                              gtatatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCtg                                                                                            <  1:3847484/98‑2 (MQ=255)  tatatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTg                                                                                           <  1:1733830/99‑1 (MQ=255)   atatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGa                                                                                          <  1:2293079/100‑1 (MQ=255)     ataAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCttagat                                                                                        <  1:2678909/100‑5 (MQ=255)           tgtaaGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATtc                                                                                  >  1:1861665/4‑100 (MQ=255)                   aTGTCTATCGCTATCACGATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATtctttatctt                                                                          <  1:5723018/100‑1 (MQ=255)                                     aTTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACAttttgt                                                        >  1:345257/1‑100 (MQ=255)                                       tGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGa                                                      >  1:2331530/1‑100 (MQ=255)                                       tGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGa                                                      >  1:5145958/1‑100 (MQ=255)                                        tccAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGaa                                                     >  1:1428089/2‑100 (MQ=255)                                        gCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGaa                                                     >  1:5329101/1‑100 (MQ=255)                                        gCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGaa                                                     >  1:3859205/1‑100 (MQ=255)                                                                        tatCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGct                     <  1:2706254/100‑1 (MQ=255)                                                                            tgtgGTGTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGcttctt                 >  1:2658032/1‑100 (MQ=255)                                                                             gtggtgTACTAACAACTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCttt                >  1:919418/1‑100 (MQ=255)                                                                                       aacaacTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCtcttct      >  1:409353/1‑100 (MQ=255)                                                                                       aacaacTGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCtcttct      >  1:1479624/1‑100 (MQ=255)                                                                                           cttGCGCCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtgatg  >  1:140410/3‑100 (MQ=255)                                                                                                 |                                                                                              GAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATG  >  NC_007793/1579384‑1579573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑