Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,416	T→C	V137V (GTA→GTG)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,416	0	T	C	100.0%	58.3 / NA	22	V137V (GTA→GTG)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/18);  total (4/18)

TCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTT  >  NC_007793/1579320‑1579501                                                                                                 |                                                                                      tCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATcaca                                                                                    <  1:4577422/100‑1 (MQ=255)        tGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCa                                                                             >  1:2009892/1‑100 (MQ=255)        ggTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCa                                                                             >  1:3832926/2‑100 (MQ=255)            tttCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAAttt                                                                         <  1:656603/100‑2 (MQ=255)                    ttCCTTTTCATTTTCTTTTTGTATGGGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCgggta                                                                 <  1:2115406/100‑3 (MQ=255)                    ttCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGAt                                                                 <  1:1915916/100‑1 (MQ=255)                     tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATa                                                                <  1:2646164/100‑1 (MQ=255)                     tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATa                                                                <  1:3549972/100‑1 (MQ=255)                             aTTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCtg                                                        <  1:3797489/100‑2 (MQ=255)                                            gCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCtgtg                                         <  1:589753/100‑1 (MQ=255)                                                cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACGATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGgtgt                                     <  1:2942162/100‑1 (MQ=255)                                                cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                     >  1:1253634/1‑100 (MQ=255)                                                cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                     <  1:5429688/100‑1 (MQ=255)                                                 aaTGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgta                                    <  1:2617567/100‑2 (MQ=255)                                                    gAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTa                                 <  1:3701064/100‑1 (MQ=255)                                                              aTAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCgc                       <  1:190237/100‑2 (MQ=255)                                                               tAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCgcc                      <  1:1149751/100‑3 (MQ=255)                                                                 gtatatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCtg                    <  1:3847484/98‑2 (MQ=255)                                                                  tatatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTg                   <  1:1733830/99‑1 (MQ=255)                                                                   atatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGa                  <  1:2293079/100‑1 (MQ=255)                                                                     ataAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCttagat                <  1:2678909/100‑5 (MQ=255)                                                                           tgtaaGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATtc          >  1:1861665/4‑100 (MQ=255)                                                                                   aTGTCTATCGCTATCACGATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGAGCAAATtctttatctt  <  1:5723018/100‑1 (MQ=255)                                                                                                 |                                                                                      TCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTT  >  NC_007793/1579320‑1579501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑