Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,396	A→T	F144Y (TTC→TAC)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,396	0	A	T	100.0%	70.1 / NA	26	F144Y (TTC→TAC)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/20);  total (6/20)

TTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGC  >  NC_007793/1579302‑1579487                                                                                               |                                                                                            ttttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCt                                                                                        >  1:915041/1‑100 (MQ=255)  tttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTa                                                                                       >  1:1148100/1‑100 (MQ=255)   ttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTAt                                                                                      <  1:3841395/100‑1 (MQ=255)      cTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTAAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTc                                                                                   <  1:5210808/100‑1 (MQ=255)            ctgctgTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGc                                                                             >  1:1299253/1‑100 (MQ=255)                   tCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATcaca                                                                      <  1:4577422/100‑1 (MQ=255)                          tGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCa                                                               >  1:2009892/1‑100 (MQ=255)                          ggTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCa                                                               >  1:3832926/2‑100 (MQ=255)                              tttCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAAttt                                                           <  1:656603/100‑2 (MQ=255)                                      ttCCTTTTCATTTTCTTTTTGTATGGGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCgggta                                                   <  1:2115406/100‑3 (MQ=255)                                      ttCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGAt                                                   <  1:1915916/100‑1 (MQ=255)                                       tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATa                                                  <  1:2646164/100‑1 (MQ=255)                                       tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATa                                                  <  1:3549972/100‑1 (MQ=255)                                               aTTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCtg                                          <  1:3797489/100‑2 (MQ=255)                                                              gCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCtgtg                           <  1:589753/100‑1 (MQ=255)                                                                  cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACGATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGgtgt                       <  1:2942162/100‑1 (MQ=255)                                                                  cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                       <  1:5429688/100‑1 (MQ=255)                                                                  cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                       >  1:1253634/1‑100 (MQ=255)                                                                   aaTGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgta                      <  1:2617567/100‑2 (MQ=255)                                                                      gAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTa                   <  1:3701064/100‑1 (MQ=255)                                                                                aTAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCgc         <  1:190237/100‑2 (MQ=255)                                                                                 tAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCgcc        <  1:1149751/100‑3 (MQ=255)                                                                                   gtatatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCtg      <  1:3847484/98‑2 (MQ=255)                                                                                    tatatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTg     <  1:1733830/99‑1 (MQ=255)                                                                                     atatAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCTTGa    <  1:2293079/100‑1 (MQ=255)                                                                                       ataAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGCCttagat  <  1:2678909/100‑5 (MQ=255)                                                                                               |                                                                                            TTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGC  >  NC_007793/1579302‑1579487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑