Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,385	A→T	S148T (TCT→ACT)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,385	0	A	T	100.0%	73.9 / NA	28	S148T (TCT→ACT)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/17);  total (11/17)

TTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTC  >  NC_007793/1579290‑1579481                                                                                                |                                                                                                 ttACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTatat                                                                                              >  1:4279777/1‑100 (MQ=255) ttACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTatat                                                                                              >  1:35024/1‑100 (MQ=255) ttACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCCTTTTGTATGCGTCAATGAGTTGGTCGATAGTatat                                                                                              >  1:3470705/1‑100 (MQ=255) cgtgtggTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCCTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTatat                                                                                              >  1:5298016/7‑100 (MQ=255)  tACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGtatata                                                                                             >  1:2173707/1‑100 (MQ=255)        tATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTgt                                                                                       <  1:1033537/100‑2 (MQ=255)        tATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTgt                                                                                       >  1:412143/1‑99 (MQ=255)             ttttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCt                                                                                  >  1:915041/1‑100 (MQ=255)              tttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTa                                                                                 >  1:1148100/1‑100 (MQ=255)               ttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTAt                                                                                <  1:3841395/100‑1 (MQ=255)                  cTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTAAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTc                                                                             <  1:5210808/100‑1 (MQ=255)                        ctgctgTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGc                                                                       >  1:1299253/1‑100 (MQ=255)                               tCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATcaca                                                                <  1:4577422/100‑1 (MQ=255)                                      tGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCa                                                         >  1:2009892/1‑100 (MQ=255)                                      ggTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCa                                                         >  1:3832926/2‑100 (MQ=255)                                          tttCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAAttt                                                     <  1:656603/100‑2 (MQ=255)                                                  ttCCTTTTCATTTTCTTTTTGTATGGGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCgggta                                             <  1:2115406/100‑3 (MQ=255)                                                  ttCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGAt                                             <  1:1915916/100‑1 (MQ=255)                                                   tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATa                                            <  1:3549972/100‑1 (MQ=255)                                                   tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATa                                            <  1:2646164/100‑1 (MQ=255)                                                           aTTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCtg                                    <  1:3797489/100‑2 (MQ=255)                                                                          gCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCtgtg                     <  1:589753/100‑1 (MQ=255)                                                                              cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACGATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGgtgt                 <  1:2942162/100‑1 (MQ=255)                                                                              cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                 >  1:1253634/1‑100 (MQ=255)                                                                              cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                 <  1:5429688/100‑1 (MQ=255)                                                                               aaTGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgta                <  1:2617567/100‑2 (MQ=255)                                                                                  gAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTa             <  1:3701064/100‑1 (MQ=255)                                                                                            aTAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCgc   <  1:190237/100‑2 (MQ=255)                                                                                             tAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCgcc  <  1:1149751/100‑3 (MQ=255)                                                                                                |                                                                                                 TTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTC  >  NC_007793/1579290‑1579481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑