Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,374	C→T	Q151Q (CAG→CAA)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,374	0	C	T	100.0%	79.6 / NA	29	Q151Q (CAG→CAA)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/16);  total (13/16)

TAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTA  >  NC_007793/1579280‑1579470                                                                                               |                                                                                                 tAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGtg                                                                                             <  1:4975236/100‑2 (MQ=255)  aaGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCg                                                                                            >  1:744690/1‑100 (MQ=255)           ttACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTatat                                                                                   >  1:4279777/1‑100 (MQ=255)           ttACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTatat                                                                                   >  1:35024/1‑100 (MQ=255)           ttACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCCTTTTGTATGCGTCAATGAGTTGGTCGATAGTatat                                                                                   >  1:3470705/1‑100 (MQ=255)           cgtgtggTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCCTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTatat                                                                                   >  1:5298016/7‑100 (MQ=255)            tACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGtatata                                                                                  >  1:2173707/1‑100 (MQ=255)                  tATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTgt                                                                            <  1:1033537/100‑2 (MQ=255)                  tATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTgt                                                                            >  1:412143/1‑99 (MQ=255)                       ttttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCt                                                                       >  1:915041/1‑100 (MQ=255)                        tttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTa                                                                      >  1:1148100/1‑100 (MQ=255)                         ttCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTAt                                                                     <  1:3841395/100‑1 (MQ=255)                            cTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTAAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTc                                                                  <  1:5210808/100‑1 (MQ=255)                                  ctgctgTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGc                                                            >  1:1299253/1‑100 (MQ=255)                                         tCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATcaca                                                     <  1:4577422/100‑1 (MQ=255)                                                tGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCa                                              >  1:2009892/1‑100 (MQ=255)                                                ggTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCa                                              >  1:3832926/2‑100 (MQ=255)                                                    tttCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAAttt                                          <  1:656603/100‑2 (MQ=255)                                                            ttCCTTTTCATTTTCTTTTTGTATGGGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCgggta                                  <  1:2115406/100‑3 (MQ=255)                                                            ttCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGAt                                  <  1:1915916/100‑1 (MQ=255)                                                             tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATa                                 <  1:2646164/100‑1 (MQ=255)                                                             tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATa                                 <  1:3549972/100‑1 (MQ=255)                                                                     aTTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCtg                         <  1:3797489/100‑2 (MQ=255)                                                                                    gCGTCAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCtgtg          <  1:589753/100‑1 (MQ=255)                                                                                        cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACGATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGgtgt      <  1:2942162/100‑1 (MQ=255)                                                                                        cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt      <  1:5429688/100‑1 (MQ=255)                                                                                        cAATGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt      >  1:1253634/1‑100 (MQ=255)                                                                                         aaTGAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgta     <  1:2617567/100‑2 (MQ=255)                                                                                            gAGTTGGTCGATAGTATATAAGTTGTAAGCTATGTCTATCGCTATCACAATTGCCAATTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTa  <  1:3701064/100‑1 (MQ=255)                                                                                               |                                                                                                 TAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTA  >  NC_007793/1579280‑1579470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑