| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,579,279 | C→A | intergenic (‑26/+11) | SAUSA300_RS07725 ← / ← SAUSA300_RS07730 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,579,279 | 0 | C | A | 88.9% | 16.7 / ‑4.1 | 9 | intergenic (‑26/+11) | SAUSA300_RS07725/SAUSA300_RS07730 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (3/5); total (3/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
TTTAGCTTTAGTTATGTGTGTATGCTTGCGTCCTGTTGAATCTTTGAATGTTGTGACTAAGTATTGCGTCACTTCCCCAAAACCTCCTTGACTCGATCTAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCT > NC_007793/1579182‑1579375 | tttAGCTTTAGTTATGTGTGTAGGCTTGCGGCCTGTTGAATCTTTAAATGTTGTGTCTAAGTATTGCGTCACTTCCCCAAAACCTCCTTGACTCGATCTa < 1:4881104/100‑1 (MQ=25) ccatgtcgctCACTTCCCCAAAACCTCCTTGACTCGATATAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGtt < 1:3721656/91‑1 (MQ=21) aaaCCTCCTTGACTCGATATAAGATGTCTTTACGCGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGt < 1:1283397/100‑1 (MQ=39) gatctGACTCGATATAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGcc < 1:3255413/96‑2 (MQ=255) cctTGACTCGATATAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGt > 1:3324940/1‑100 (MQ=255) cctTGACTCGATATAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCCTTTTGTATGCGt > 1:2884837/1‑100 (MQ=255) gACTCGATATAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAAt > 1:4762511/1‑100 (MQ=255) aCTCGATATAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATg < 1:5085023/100‑1 (MQ=255) gATATAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAgtt < 1:72134/100‑3 (MQ=255) | TTTAGCTTTAGTTATGTGTGTATGCTTGCGTCCTGTTGAATCTTTGAATGTTGTGACTAAGTATTGCGTCACTTCCCCAAAACCTCCTTGACTCGATCTAAGATGTCTTTACACGTATCCTTTTCCTGCGTCTGCTGTTCCATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCT > NC_007793/1579182‑1579375 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |