Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,544,669	C→A	M1M (ATG→ATT) †	SAUSA300_RS07525 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,544,669	0	C	A	100.0%	45.7 / NA	17	M1M (ATG→ATT) †	SAUSA300_RS07525	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/17);  total (0/17)

TTCTGTATTTGTGTCTTTTTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAACATAGCTCATCACCCATTTATGTGTTTATATAGAATATTAAAAAGCATTTT‑AAAAAAATTTATTCATCTTTACGATACAC  >  NC_007793/1544576‑1544748                                                                                              |                                                                                 ttctgtatttgtGTCTTTTTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCt                                                                            <  1:3503964/100‑1 (MQ=255)       atttgtGTCTTTTTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCAc                                                                      <  1:1558446/100‑1 (MQ=255)         ttgtGTCTTTTTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCGTCAccc                                                                    <  1:4330754/100‑1 (MQ=255)           tccgatctttttGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCag                                                                  <  1:267450/93‑2 (MQ=25)           gtgtCTTTTTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCaa                                                                  <  1:5698684/100‑2 (MQ=255)           gtgtCTTTTTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCAt                                                                  <  1:2108444/100‑1 (MQ=255)            tgtgtctttttGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCAta                                                                 <  1:1547003/94‑2 (MQ=255)             gtCTTTTTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCAttt                                                                <  1:938770/100‑1 (MQ=255)             gtCTTTCTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCAttt                                                                <  1:4945459/100‑1 (MQ=255)             cttccgatctGATTCGTTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCAttt                                                                <  1:629427/91‑1 (MQ=21)               cTTTTTTTAATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCATTTAt                                                              <  1:3135035/100‑1 (MQ=255)                    tttGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCATTTATGTGtt                                                         <  1:4651269/100‑1 (MQ=255)                     ttGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCATTTATGTGttt                                                        <  1:1276415/100‑1 (MQ=255)                      tGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCATTTATGTGtttt                                                       <  1:1008785/100‑2 (MQ=255)                         ttCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCATTTATGTGTTtatat                                                    <  1:1616826/100‑1 (MQ=255)                           cATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAAAATAGCTCATCACCCATTTATGTGTTTATATAg                                                  <  1:5402180/100‑1 (MQ=255)                                                                           ttAATTTCGCCTTTTTAAAAATAGCTCATCACCCATTTATGTGTTTATATAGAATATTAAAAAGCATTTTAAAAAAAATTTATTCATCTTTACGATacag  <  1:3367494/100‑2 (MQ=255)                                                                                              |                                                                                 TTCTGTATTTGTGTCTTTTTTTGATTCATTATTCATCGCTGAACATCCTGATAACAGTAGCGTTGCTATTAAGATTAATTTCGCCTTTTTAAACATAGCTCATCACCCATTTATGTGTTTATATAGAATATTAAAAAGCATTTT‑AAAAAAATTTATTCATCTTTACGATACAC  >  NC_007793/1544576‑1544748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑