Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,535,297	G→A	A235T (GCG→ACG)	SAUSA300_RS07470 →	L‑asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,535,297	0	G	A	100.0%	153.4 / NA	55	A235T (GCG→ACG)	SAUSA300_RS07470	L‑asparaginase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/25);  total (30/25)

AACTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAGCGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTGCTAGTTTCACGTTCCTTT  >  NC_007793/1535202‑1535392                                                                                                |                                                                                                aaCTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGtt                                                                                             <  1:5468262/100‑1 (MQ=255) aaCTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGtt                                                                                             <  1:5406274/100‑1 (MQ=255)  aCTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTa                                                                                            <  1:5325981/100‑1 (MQ=255)         gTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAg                                                                                     >  1:4590307/1‑100 (MQ=255)         gTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACATTAAGatcgg                                                                                     >  1:4975479/1‑96 (MQ=255)           aCCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGc                                                                                   <  1:3364045/100‑1 (MQ=255)           aCCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGc                                                                                   <  1:1757399/100‑1 (MQ=255)            ccATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCa                                                                                  <  1:934619/100‑1 (MQ=255)            ccATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCa                                                                                  <  1:5123463/100‑1 (MQ=255)            ccATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAAGACAAGGCa                                                                                  <  1:668513/100‑1 (MQ=255)              aTTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCaaa                                                                                <  1:4361631/100‑2 (MQ=255)                   ttaaaGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATAcc                                                                           >  1:865460/3‑100 (MQ=255)                    aaaaGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCt                                                                          >  1:5776594/1‑100 (MQ=255)                      caGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTcc                                                                        >  1:304608/2‑100 (MQ=255)                      aaGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTcc                                                                        >  1:5768020/1‑100 (MQ=255)                         cATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAg                                                                     >  1:2961106/1‑100 (MQ=255)                           cacctGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAgcg                                                                   >  1:4383633/5‑100 (MQ=255)                            atatGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAgcgc                                                                  >  1:5139498/1‑100 (MQ=255)                            atatGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAgcag                                                                  >  1:2981018/1‑98 (MQ=255)                             catGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCa                                                                 >  1:3221553/2‑100 (MQ=255)                             aatGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCa                                                                 >  1:4385780/2‑100 (MQ=255)                              atGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAgcgctt                                                                <  1:5209729/100‑3 (MQ=255)                              atGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCaa                                                                <  1:4021740/100‑2 (MQ=255)                              atGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCAt                                                                <  1:4227276/100‑1 (MQ=255)                              atGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCCCCAAgatcgg                                                                >  1:269321/1‑95 (MQ=255)                               tGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCAtt                                                               <  1:2077852/100‑1 (MQ=255)                               tGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCAtt                                                               <  1:4340213/100‑1 (MQ=255)                               tGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCAtt                                                               <  1:2213329/100‑1 (MQ=255)                               tGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCAtt                                                               <  1:2620999/100‑1 (MQ=255)                               tGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCAtt                                                               <  1:4966021/100‑1 (MQ=255)                                  atATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAgcaagatcgg                                                            >  1:4962504/2‑92 (MQ=255)                                   tATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCagatcgg                                                           >  1:5685972/1‑94 (MQ=255)                                    gtGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAACGCagatcgga                                                          >  1:2910835/2‑93 (MQ=255)                                    aTGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGGCAAGGCAACATACCTCCAAGCGCaagatcgg                                                          >  1:2045597/1‑93 (MQ=255)                                    aTGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAgcgcgagatcgg                                                          >  1:2784105/1‑92 (MQ=255)                                    aTGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCacagatcg                                                          >  1:3553727/1‑93 (MQ=255)                                    aTGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCacagatcg                                                          >  1:4922867/1‑93 (MQ=255)                                    aTGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCaagatcgg                                                          >  1:3756722/1‑93 (MQ=255)                                    aTGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAg                                                          >  1:2664567/1‑100 (MQ=255)                                      gCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCAtagatcgga                                                        >  1:2247609/1‑92 (MQ=255)                                      gCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGc                                                        >  1:463535/1‑100 (MQ=255)                                       ccAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTGTTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGcc                                                       <  1:4512935/100‑2 (MQ=255)                                       ccAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCa                                                       >  1:150986/1‑100 (MQ=255)                                        cAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCAt                                                      >  1:1821197/1‑100 (MQ=255)                                        cAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATCAGatcggaa                                                      >  1:431574/1‑94 (MQ=255)                                           gTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGTTATGGTTATTGAAACTTTATTACAAGGCAACATACCTCCAAGCGCATTAAAATGCATTca                                                   <  1:4141187/100‑1 (MQ=255)                                           gTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTca                                                   <  1:2120285/100‑1 (MQ=255)                                                  ttttAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTa                                            <  1:71409/100‑1 (MQ=255)                                                         ttttATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCtt                                     <  1:2693444/100‑1 (MQ=255)                                                            tataGTCGAGAAGGTATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTaa                                  >  1:888583/1‑100 (MQ=255)                                                                      aaGGCATCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTAt                        >  1:2951564/1‑100 (MQ=255)                                                                            tCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTGCt                  <  1:2553645/100‑1 (MQ=255)                                                                            tCGATGGTATGGTTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTGCt                  <  1:1030925/100‑1 (MQ=255)                                                                                      ggtTATTGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTGCTAGTTTCACGt        >  1:477759/1‑100 (MQ=255)                                                                                            tGAAACGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTGCTAGTTTCACGTTCCttt  <  1:3653303/100‑1 (MQ=255)                                                                                                |                                                                                                AACTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTTAGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAGCGTTAGGACAAGGCAACATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTGCTAGTTTCACGTTCCTTT  >  NC_007793/1535202‑1535392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑