Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 1,534,172 G→T A116D (GCT→GAT)  SAUSA300_RS07465 ← cytidylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077931,534,1720GT99.4% 462.8 / NA 163A116D (GCT→GAT) SAUSA300_RS07465cytidylate kinase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base T (7/154);  minor base A (0/1);  total (8/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

CGCTCTTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCAGCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCACATCATTATTTCTTAAAAAGT  >  NC_007793/1534073‑1534268
                                                                                                   |                                                                                                
cgctcTTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCAg                                                                                                  <  1:521771/100‑1 (MQ=255)
  ctctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCt                                                                                                <  1:8133/100‑1 (MQ=255)
  ctctTCAACTGATGCAATCATATATACTGTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCt                                                                                                <  1:4849427/100‑1 (MQ=255)
  ctctTCAACTGACACAATCATATATACATTTAAATCTACATCTGGTAGCACCACAGTTCCGATATCGCGACCAACCATAACGATACCTTTTACTGCATCt                                                                                                <  1:1297069/100‑1 (MQ=255)
   ttccgatcTGATGCTATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:5077044/93‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCGTCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:538678/100‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:1878336/100‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:2130978/100‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:2101268/100‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:3402061/100‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:3478696/100‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:4324427/100‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:1269435/100‑1 (MQ=255)
   tctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCAACCATCCATTACGATACCTTTTTCTGCATCTa                                                                                               <  1:3288836/100‑1 (MQ=255)
    tccgatcTGGTGTAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTaa                                                                                              <  1:5031652/94‑1 (MQ=255)
    tccgatcTGATGCGATCACATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTag                                                                                              <  1:1878577/94‑2 (MQ=255)
    ctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTag                                                                                              <  1:3813178/100‑2 (MQ=255)
    ctTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTaa                                                                                              <  1:3031894/100‑1 (MQ=255)
      tCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTagat                                                                                            <  1:2976638/100‑4 (MQ=255)
      tCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAAct                                                                                            <  1:3529574/100‑1 (MQ=255)
       cAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAActc                                                                                           >  1:4893535/1‑100 (MQ=255)
           tGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCtttg                                                                                       <  1:3278863/100‑2 (MQ=255)
             aTGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTg                                                                                     <  1:2724065/100‑1 (MQ=255)
              tGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGt                                                                                    <  1:269518/100‑1 (MQ=255)
               gCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGtt                                                                                   <  1:1678860/100‑1 (MQ=255)
                cAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGttc                                                                                  <  1:1381580/100‑2 (MQ=255)
                   tCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGtttttt                                                                               <  1:2458428/100‑1 (MQ=255)
                   tCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGtttttt                                                                               <  1:2681810/100‑1 (MQ=255)
                        tataCTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCGTCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACgg                                                                          <  1:4637382/100‑1 (MQ=255)
                          taCTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGca                                                                        <  1:254518/100‑2 (MQ=255)
                           aCTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGctt                                                                       <  1:4657908/100‑3 (MQ=255)
                           aCTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGa                                                                       <  1:961548/100‑1 (MQ=255)
                           aCTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGa                                                                       <  1:1700891/100‑1 (MQ=255)
                           aCTGTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGa                                                                       <  1:1942616/100‑1 (MQ=255)
                            cTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGgagat                                                                      <  1:716486/100‑2 (MQ=255)
                            cTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGaa                                                                      <  1:109624/100‑1 (MQ=255)
                              tttAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCCGCATCTAACTCTTTTTGTTTTTTAACGGCGAATg                                                                    <  1:4164412/100‑1 (MQ=255)
                              ttccgaTCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATg                                                                    <  1:5048109/95‑1 (MQ=255)
                                  aaTCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGagca                                                                <  1:567577/100‑4 (MQ=255)
                                   gcttGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAAAGATCgg                                                               >  1:4629384/4‑99 (MQ=255)
                                       gCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTg                                                           <  1:1462994/100‑1 (MQ=255)
                                       gCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTg                                                           <  1:1231146/100‑1 (MQ=255)
                                         aTCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTggg                                                         <  1:2802464/100‑2 (MQ=255)
                                         aTCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTgat                                                         <  1:2428266/100‑3 (MQ=255)
                                         aTCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGc                                                         <  1:3862950/100‑1 (MQ=255)
                                          tCTGGTAGCACTCCAGTTCCGATATCGCGACCATCCATAACGATACAGTGATCCGCAACTAAATCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGct                                                        <  1:3435604/100‑1 (MQ=255)
                                          tCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTggtg                                                        <  1:740554/100‑3 (MQ=255)
                                          tCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGct                                                        <  1:4980553/100‑1 (MQ=255)
                                          tCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGct                                                        <  1:4937047/100‑1 (MQ=255)
                                          tCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGct                                                        <  1:1534295/100‑1 (MQ=255)
                                          tCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGct                                                        <  1:2058339/100‑1 (MQ=255)
                                          tCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGct                                                        <  1:3562095/100‑1 (MQ=255)
                                          tCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGcc                                                        <  1:2644162/100‑2 (MQ=255)
                                           cTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGctt                                                       <  1:906622/100‑2 (MQ=255)
                                           cTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGctt                                                       <  1:1042716/100‑2 (MQ=255)
                                           cTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGctg                                                       <  1:223631/100‑2 (MQ=255)
                                           cTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGctg                                                       <  1:2573805/100‑2 (MQ=255)
                                           cTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGcta                                                       <  1:1924567/100‑2 (MQ=255)
                                           cTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGcta                                                       <  1:5099441/100‑2 (MQ=255)
                                            tGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGctat                                                      <  1:914873/100‑3 (MQ=255)
                                             ggTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCtt                                                     >  1:1197231/1‑100 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGGCCATCCATTACGATGCCTTTTTCTGCATCTAACTCTTTTAGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:1060424/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATTCCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATTAACGTACTGGCTCttt                                                    <  1:4476807/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATTCCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:4126357/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:5616560/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:5066839/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:3198878/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:5103693/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:5343627/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:5406427/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:544010/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:3249872/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:639674/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:2925207/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:2861243/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:5022772/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:3781833/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:4020368/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:2196958/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:3630997/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:336020/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:4607524/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:155879/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:1627063/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:4653704/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTCTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:307272/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACGACACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:328132/100‑1 (MQ=255)
                                              gTAGCACTACAGTTCCGATATCGCGACCATCCATTACAATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCttt                                                    <  1:4729485/100‑1 (MQ=255)
                                                    cTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGc                                              <  1:4917056/100‑1 (MQ=255)
                                                     tACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCa                                             <  1:5430939/100‑1 (MQ=255)
                                                           tCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACggca                                       <  1:518851/100‑4 (MQ=255)
                                                                       aCCATCCATTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGc                           <  1:2957204/100‑1 (MQ=255)
                                                                         catccatTACGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGt                         <  1:3169269/100‑1 (MQ=255)
                                                                                 aCGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCAcatcac                 <  1:4470409/100‑2 (MQ=255)
                                                                                  cGATACCTTTTTCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCACATCatt                <  1:5534563/100‑1 (MQ=255)
                                                                                        cTTTTTCTGCATCTAACTC‑TTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCACATCATTATTTCtt         <  1:3709810/100‑1 (MQ=255)
                                                                                         tttttCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCACATCATTATTTCtt         <  1:5229837/100‑1 (MQ=255)
                                                                                         tttttCTGCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCACATCATTATTTCtt         <  1:1708687/100‑1 (MQ=255)
                                                                                                gCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCACATCATTATTTCTTAAAAAGt  >  1:1586380/1‑100 (MQ=255)
                                                                                                gCATCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCACATCATTATTTCTTAAAAAGt  >  1:4943299/1‑100 (MQ=255)
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CGCTCTTCAACTGATGCAATCATATATACTTTTAAATCTGCATCTGGTAGCACTACAGTTCCGATATCGCGACCATCCATTACGATACCTTTTTCTGCAGCTAACTCTTTTTGTTTTTTAACGGCGAATGAACGTACTGGCTCTTTAGATGCAACGTATGAAACATGTTGCGTCACATCATTATTTCTTAAAAAGT  >  NC_007793/1534073‑1534268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑