Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,499,825	G→T	G361C (GGC→TGC)	SAUSA300_RS07270 →	sulfite reductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,499,825	0	G	T	100.0%	27.9 / NA	12	G361C (GGC→TGC)	SAUSA300_RS07270	sulfite reductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/6);  total (6/6)

AAAAAGCTAGGTACGATTAAATATGATGACTACGGTATTATTGCTAAATGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTCGGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAA  >  NC_007793/1499735‑1499907                                                                                           |                                                                                   aaaaaGCTAGGTACGATTAAATATGATGACTACGGTATTATTGCTAAATGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTCTGCATTTtaa                                                                           >  1:5287260/1‑100 (MQ=255)                  tAAATATGATGACTACGGTATTATTGCTAAATGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTCTGCATTTTAAATAAAATCATTAattta                                                          >  1:1221409/1‑100 (MQ=255)                                aCGGTATTATTGCTAAATGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGc                                            >  1:1446567/1‑100 (MQ=255)                                              aaaTGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCa                              >  1:1046669/1‑100 (MQ=255)                                                     ttACATGGGGCATTCCGCTTCACGAAGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCAt                       >  1:5613635/1‑100 (MQ=255)                                                      tACATGGGGCATTCCGCTTCACGAAGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTAGTTGTATGTATCGCTTTATTAGTAGCCATTGGCATg                      >  1:4602871/1‑100 (MQ=255)                                                            ctcttccgatcTTCGCGGAGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGttt                <  1:2863288/90‑1 (MQ=255)                                                               ttccgatcTTCACGAAGGTAATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGtc             <  1:3739913/93‑1 (MQ=255)                                                                ttccgatctCACGACGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGtct            <  1:3113530/92‑1 (MQ=255)                                                                     tcTTCGCGAAGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTgg       <  1:2266861/99‑1 (MQ=255)                                                                     atctCACGATGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTgg       <  1:1104650/97‑1 (MQ=255)                                                                          aCGAAGGTCATTTATTCTGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATatg  <  1:3680076/100‑3 (MQ=255)                                                                                           |                                                                                   AAAAAGCTAGGTACGATTAAATATGATGACTACGGTATTATTGCTAAATGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTCGGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAA  >  NC_007793/1499735‑1499907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑