Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,499,394	C→A	A217D (GCC→GAC)	SAUSA300_RS07270 →	sulfite reductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,499,394	0	C	A	100.0%	53.4 / NA	19	A217D (GCC→GAC)	SAUSA300_RS07270	sulfite reductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/1);  total (18/1)

TCCAGTATTTGTATTAGTCTTAACTGGATTGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGCCCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTAAAAATAAACAGCCAGCTTCATCCG  >  NC_007793/1499308‑1499489                                                                                       |                                                                                                tCCAGTATTTGTATTAGTCTTAACTGGATTGCCATGGTCTGGTTTTATGGGCAGT‑AAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTg                                                                                   <  1:1936573/100‑1 (MQ=255)  ccAGTATTTGTATTAGTCTTAACTGGATTGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTg                                                                                   >  1:2117824/1‑100 (MQ=255)           gTATTAGTCTTAACTGGATTGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGa                                                                          >  1:4231474/1‑100 (MQ=255)                     tAACTGGATTGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCAcc                                                                >  1:33220/1‑100 (MQ=255)                         tGGATTGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTaaa                                                            >  1:3501080/1‑100 (MQ=255)                          ggATTGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAAt                                                           >  1:2779924/1‑100 (MQ=255)                          ggATTGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAAt                                                           >  1:482359/1‑100 (MQ=255)                              tGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGa                                                       >  1:4608968/1‑100 (MQ=255)                                ccATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGAtt                                                     >  1:2510633/1‑100 (MQ=255)                                         ggTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAAt                                            >  1:4505135/1‑100 (MQ=255)                                               aTGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCtt                                      >  1:4476086/1‑100 (MQ=255)                                                           ctagCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTa                          >  1:3410214/4‑100 (MQ=255)                                                             tGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTaaa                        >  1:5570966/1‑100 (MQ=255)                                                             tGCCGGTATGATGGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTaaa                        >  1:1334138/1‑100 (MQ=255)                                                                      tatgGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTAAAAATAAAcag               >  1:1520917/2‑100 (MQ=255)                                                                      gatgGACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTAAAAATAAAcag               >  1:581933/1‑100 (MQ=255)                                                                          gACACAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTAAAAATAAAcagccag           >  1:4037542/1‑100 (MQ=255)                                                                           acacAAACGGTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTAAAAATAAAcagccaga          >  1:937360/1‑99 (MQ=255)                                                                                   tgTGACCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTAAAAATAAACAGCCAGCTTCATCCg  >  1:959790/2‑100 (MQ=255)                                                                                       |                                                                                                TCCAGTATTTGTATTAGTCTTAACTGGATTGCCATGGTCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGCCCTTGGTCAAGGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGTAAAAATAAACAGCCAGCTTCATCCG  >  NC_007793/1499308‑1499489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑