Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,495,206	T→C	E1079G (GAA→GGA)	SAUSA300_RS07265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,495,206	0	T	C	100.0%	138.5 / NA	49	E1079G (GAA→GGA)	SAUSA300_RS07265	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/48);  total (1/48)

AACTTTCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATTCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGC  >  NC_007793/1495114‑1495301                                                                                             |                                                                                                aaCTTTCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGt                                                                                          <  1:3359943/100‑1 (MQ=255)    tttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTTTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACt                                                                                       <  1:2868507/100‑1 (MQ=255)    tttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTAcc                                                                                       <  1:538908/100‑2 (MQ=255)    tttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTAca                                                                                       <  1:5224481/100‑2 (MQ=255)    tttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACt                                                                                       <  1:3180329/100‑1 (MQ=255)     ttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACtt                                                                                      <  1:5010560/100‑1 (MQ=255)            tGCGGTTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACaag                                                                               <  1:525408/100‑2 (MQ=255)            tGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACaag                                                                               <  1:1693224/100‑2 (MQ=255)                      cTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATg                                                                     <  1:290409/100‑1 (MQ=255)                              gCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATAtctt                                                             <  1:3153131/100‑2 (MQ=255)                                     gCTTTACATATAATTCAAGCTGTTGCCTGAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGccc                                                      <  1:3408547/100‑1 (MQ=255)                                     gCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGccc                                                      <  1:1524294/100‑1 (MQ=255)                                     gCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGccc                                                      <  1:539192/100‑1 (MQ=255)                                     gCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGccc                                                      <  1:2149381/100‑1 (MQ=255)                                      cTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCa                                                     <  1:1535345/100‑1 (MQ=255)                                       tttACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGccaga                                                    <  1:4742855/100‑4 (MQ=255)                                       tttACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCag                                                    <  1:5432784/100‑2 (MQ=255)                                       tttACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCAt                                                    <  1:5655947/100‑1 (MQ=255)                                       tttACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCAt                                                    <  1:3856199/100‑1 (MQ=255)                                        ttACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCagg                                                   <  1:1558991/100‑3 (MQ=255)                                        ttACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCAtt                                                   <  1:1629477/100‑1 (MQ=255)                                         tACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCAttg                                                  <  1:4489410/100‑2 (MQ=255)                                           tgtgctttccAATCTATTGCCTCAATAATCCCATTTGTGATTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTgg                                                <  1:135305/91‑1 (MQ=255)                                           cATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTgg                                                <  1:5707846/100‑1 (MQ=255)                                           cATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTgg                                                <  1:3406869/100‑1 (MQ=255)                                           cATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAAACCTAACGTGCTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTgg                                                <  1:1751216/100‑1 (MQ=255)                                             tataATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGAt                                              <  1:4007832/100‑1 (MQ=255)                                                cttccgatcTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCa                                           <  1:3926541/92‑1 (MQ=255)                                                aaTTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCa                                           <  1:1043405/100‑1 (MQ=255)                                                aaTTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCa                                           <  1:843645/100‑1 (MQ=255)                                                          gTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGTCCATTTGGATTCAAAATTTTACg                                 >  1:3795880/1‑100 (MQ=255)                                                                gtgctcttCCGATCTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTgg                           <  1:1827632/93‑2 (MQ=255)                                                                         gatcttGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGttt                  <  1:3171339/96‑1 (MQ=255)                                                                         gatcttGGGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGttt                  <  1:3541402/96‑1 (MQ=255)                                                                         ccATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGttt                  <  1:5064569/100‑1 (MQ=255)                                                                         ccATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGttt                  <  1:3654917/100‑1 (MQ=255)                                                                           aTTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAg                <  1:3923035/100‑1 (MQ=255)                                                                             ttatgGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGc              <  1:2667119/97‑1 (MQ=255)                                                                                 ggTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAAta          <  1:3687962/100‑2 (MQ=255)                                                                                  gTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGc         <  1:4044308/100‑1 (MQ=255)                                                                                     gTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCAtt      <  1:4075783/100‑1 (MQ=255)                                                                                      taataaTCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCAttt     <  1:3187673/100‑1 (MQ=255)                                                                                      taataaTCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCAttt     <  1:1935868/100‑1 (MQ=255)                                                                                         taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:408163/100‑1 (MQ=255)                                                                                         taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:5192733/100‑1 (MQ=255)                                                                                         taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:826146/100‑1 (MQ=255)                                                                                         taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:1507210/100‑1 (MQ=255)                                                                                         taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:907970/100‑1 (MQ=255)                                                                                         taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCACATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:1662145/100‑1 (MQ=255)                                                                                             |                                                                                                AACTTTCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATTCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGC  >  NC_007793/1495114‑1495301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑