Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,490,274	T→C	L357L (TTA→TTG)	SAUSA300_RS07245 ←	amino acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,490,274	0	T	C	100.0%	19.5 / NA	9	L357L (TTA→TTG)	SAUSA300_RS07245	amino acid permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/7);  total (2/7)

GTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTG  >  NC_007793/1490180‑1490336                                                                                               |                                                               gTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCGACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACat                                                           <  1:5375902/100‑1 (MQ=255) gTACTTTATATGGTCGTTCCATATGTGGTACACGTTTTCTTAAAATTATGACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACat                                                           <  1:4407406/100‑1 (MQ=255)                  tCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCaa                                          <  1:2407981/100‑1 (MQ=255)                              tCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTg                              <  1:5162062/100‑1 (MQ=255)                               cACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCacaag                             <  1:455464/100‑5 (MQ=255)                                   ttttCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGAcatc                         <  1:3418616/100‑1 (MQ=255)                                     ttCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGAcatcag                       <  1:2271667/100‑2 (MQ=255)                                                   aCCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATta         >  1:3372702/1‑100 (MQ=255)                                                          aaaaTGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTg  >  1:3121676/2‑100 (MQ=255)                                                                                               |                                                               GTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTG  >  NC_007793/1490180‑1490336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑