Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,469,241	A→G	I6279T (ATT→ACT)	SAUSA300_RS07235 ←	hyperosmolarity resistance protein Ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,469,241	0	A	G	100.0%	16.1 / NA	8	I6279T (ATT→ACT)	SAUSA300_RS07235	hyperosmolarity resistance protein Ebh
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/8);  total (0/8)

TTTTGAAGTTGACCCATTGCTGTATTTAATTCATTAGCAGTATTTTGTGCAGCAGTTACGCCTGCTACTGTCGTTGCATTGTCAATTTGACTAATTGCTGCAGTTGATTGCGCATTATTCAAGTTACTATATGAAGCTAATGCACGTTTCGCTGACTCTTTTGCTTGAGCAACTTTATTATCACCAT  >  NC_007793/1469148‑1469334                                                                                              |                                                                                              ttttGAAGTTGACCCATTGCTGTATTTAATTCATTAGCAGTATTTTGTGCAGCAGTTACGCCTGCTACTGTCGTTGCATTGTCAATTTGACTAGTtgctg                                                                                         <  1:4739787/100‑1 (MQ=255)  tttGAAGTTGACCCATTGCTGTATTTAATTCATTAGCAGTATTTTGTGCAGCAGTTACGCCTGCTACTGTCGTTGCATTGTCAATTTGACTAGTtgctgc                                                                                        <  1:1165033/100‑1 (MQ=255)                                                       gTTACGCCTGCTACTGTCGTTGCATTGTCAATTTGACTAGTTGCTGCAGTTGATTGCGCATTATTCAAGTTACTATATGAAGCTAATGCACGTTTCGCTg                                   <  1:5335678/100‑1 (MQ=255)                                                         tACGCCTGCTACTGTCGTTGCATTGTCAATTTGACTAGTTGCTGCAGTTGATTGCGCATTATTCAAGTTACTATATGAAGCTAATGCACGTTTCGCTGat                                 <  1:1923615/100‑2 (MQ=255)                                                          aCGCCTGCTACTGTCGTTGCATTGTCAATTTGACTAGTTGCTGCAGTTGATTGCGCATTATTCAAGTTACTATATGAAGCTAATGCACGTTTCGCTGAca                                <  1:898432/100‑2 (MQ=255)                                                                    cTGTCGTTGCATTGTCAATTTGACTAGTTGCTGCAGTTGATTGCGCATTATTCAAGTTACTATATGAAGCTAATGCACGTTTCGCTGACTCTTTTGCTTg                      <  1:2956885/100‑1 (MQ=255)                                                                                      tttGACTAGTTGCTGCAGTTGATTGCGCATTATTCAAGTTACTATATGAAGCTAATGCACGTTTCGCTGACTCTTTTGCTTGAGCAACTTTATTATCAcc    <  1:3272854/100‑1 (MQ=255)                                                                                        tGACTAGTTGCTGCAGTTGATTGCGCATTATTCAAGTTACTATATGAAGCTAATGCACGTTTCGCTGACTCTTTTGCTTGAGCAACTTTATTATCACCAt  <  1:4267003/100‑1 (MQ=255)                                                                                              |                                                                                              TTTTGAAGTTGACCCATTGCTGTATTTAATTCATTAGCAGTATTTTGTGCAGCAGTTACGCCTGCTACTGTCGTTGCATTGTCAATTTGACTAATTGCTGCAGTTGATTGCGCATTATTCAAGTTACTATATGAAGCTAATGCACGTTTCGCTGACTCTTTTGCTTGAGCAACTTTATTATCACCAT  >  NC_007793/1469148‑1469334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑