Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,843	G→T	I226I (ATC→ATA)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,843	0	G	T	100.0%	74.4 / NA	28	I226I (ATC→ATA)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/24);  total (4/24)

GTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAAAGGGTTAGCTAAGATGTATGTGATTGAACTAATACTGAAAGGTTTCCCCTTTTTAGTGACATATCCTTTGTGATTCAATGCATTGGCAATTTTACGATAGCCATGTCCT  >  NC_007793/46755‑46939                                                                                         |                                                                                                 gTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGAt                                                                                       <  1:3060387/100‑1 (MQ=255) gTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGAt                                                                                       <  1:4188836/100‑1 (MQ=255)                          tttttCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTaccc                                                              <  1:4330489/100‑1 (MQ=255)                                      aTCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGAct                                                  <  1:3527879/100‑2 (MQ=255)                                          ttGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACatagc                                              >  1:2417541/1‑98 (MQ=255)                                          ttGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACatagc                                              >  1:2784622/1‑98 (MQ=255)                                            gTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCt                                            <  1:3098719/100‑1 (MQ=255)                                             tATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGccca                                           <  1:3563574/100‑3 (MQ=255)                                             tATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCtt                                           <  1:2232301/100‑1 (MQ=255)                                             tATTTCGCAAATTGAATATTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGTACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCtt                                           <  1:1040591/100‑1 (MQ=255)                                              aTTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATAGATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCtaa                                          <  1:2682796/100‑3 (MQ=255)                                               tttCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTCCCCTTTTTAGTGACATAGCCttta                                         <  1:2269635/100‑2 (MQ=255)                                               tttCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCttag                                         <  1:3057996/100‑3 (MQ=255)                                               tttCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtg                                         <  1:2783447/100‑1 (MQ=255)                                               tttCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtg                                         <  1:4552623/100‑1 (MQ=255)                                                 tCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtgtg                                       <  1:1670947/100‑1 (MQ=255)                                                 tCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtgtg                                       <  1:1524195/100‑1 (MQ=255)                                                 tCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtgtg                                       <  1:3248582/100‑1 (MQ=255)                                                   gCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtgtgta                                     <  1:365728/100‑3 (MQ=255)                                                   gCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGAt                                     <  1:3095528/100‑1 (MQ=255)                                                     aaaTTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTc                                   <  1:5437303/100‑1 (MQ=255)                                                          gAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGc                              <  1:3454481/100‑1 (MQ=255)                                                             ttttGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCaat                           <  1:1319408/100‑3 (MQ=255)                                                             ttttGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCAtg                           <  1:3002794/100‑2 (MQ=255)                                                                tGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGc                        <  1:3934308/100‑1 (MQ=255)                                                                      tataGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTAtttt                  >  1:3461329/1‑100 (MQ=255)                                                                         aGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACg               >  1:1110348/1‑100 (MQ=255)                                                                                      tAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACGATAGCCATGTCCt  <  1:2606793/100‑1 (MQ=255)                                                                                         |                                                                                                 GTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAAAGGGTTAGCTAAGATGTATGTGATTGAACTAATACTGAAAGGTTTCCCCTTTTTAGTGACATATCCTTTGTGATTCAATGCATTGGCAATTTTACGATAGCCATGTCCT  >  NC_007793/46755‑46939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑