Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,464,620	T→C	T7819T (ACA→ACG)	SAUSA300_RS07235 ←	hyperosmolarity resistance protein Ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,464,620	0	T	C	100.0%	53.2 / NA	22	T7819T (ACA→ACG)	SAUSA300_RS07235	hyperosmolarity resistance protein Ebh
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/22);  total (0/22)

GCATTGTTATATGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAATGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACAAGTTCTGCATGCGACACTTGGTCTTTTA  >  NC_007793/1464528‑1464708                                                                                             |                                                                                         gCATTGTTATATGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACCGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCa                                                                                   <  1:1474644/100‑1 (MQ=255)    ttGTTATATGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCAtta                                                                                <  1:593329/100‑2 (MQ=255)     tGTTATATGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGc                                                                               <  1:2986538/100‑1 (MQ=255)     tGTTATATGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGc                                                                               <  1:762205/100‑1 (MQ=255)     tGTTATATGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGc                                                                               <  1:2971440/100‑1 (MQ=255)     tGTTATATGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGc                                                                               <  1:1584986/100‑1 (MQ=255)        tataTGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCatt                                                                            <  1:1574176/100‑1 (MQ=255)         atatGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCattg                                                                           <  1:5104977/100‑2 (MQ=255)                       tgtCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGc                                                             <  1:1227553/100‑1 (MQ=255)                                  ttctgtgTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTag                                                  <  1:4975815/97‑2 (MQ=255)                                  gCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTaa                                                  <  1:3044678/100‑1 (MQ=255)                                   gctgtgTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAAt                                                 <  1:3909248/98‑1 (MQ=255)                                   cTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAAt                                                 <  1:3694241/100‑1 (MQ=255)                                                   tgactgTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTCCCGCATCAGCATTTTGCTTAATAGCATTAACACCTGtc                                 <  1:1685900/100‑2 (MQ=255)                                                      ctgTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACa                              <  1:1263064/100‑1 (MQ=255)                                                              cTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACAAGTTCtag                      <  1:1556129/100‑3 (MQ=255)                                                              cTTGACTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACAAGTTCTGc                      <  1:3213466/100‑1 (MQ=255)                                                                  aCTGTTCGCTTGAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACAAGTTCTGCATgt                  <  1:2791503/100‑2 (MQ=255)                                                                   cTGTTCGCTTGAATTTGTTGCTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACAAGTTCTGCATGCg                 <  1:2293566/100‑1 (MQ=255)                                                                             gAATTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTACTTAATAGCATTAACACCTGTAACAAGTTCTGCGTGCGACACTTGGta       <  1:5056590/100‑2 (MQ=255)                                                                               aTTTGTTGTTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACAAGTTCTGCATGCGACACTTGGTCtt     <  1:247052/100‑1 (MQ=255)                                                                                  tgttgtTTCAACGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACAAGTTCTGCATGCGACACTTGGTCTTTTa  <  1:4261268/100‑1 (MQ=255)                                                                                             |                                                                                         GCATTGTTATATGCTTGTTGTTTGTCTTGATCCGCTTGTGTAAAGTCAACTGACTGTGGTACTTGACTGTTCGCTTGAATTTGTTGTTTCAATGTACCCATTGCATTATTTAACGCATCAGCATTTTGCTTAATAGCATTAACACCTGTAACAAGTTCTGCATGCGACACTTGGTCTTTTA  >  NC_007793/1464528‑1464708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑