Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,398,667	A→G	H114R (CAT→CGT)	SAUSA300_RS06895 →	aminoacyltransferase FemB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,398,667	0	A	G	100.0%	145.7 / NA	55	H114R (CAT→CGT)	SAUSA300_RS06895	aminoacyltransferase FemB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/11);  total (44/11)

ATTTAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCATCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAGCATCATGGC  >  NC_007793/1398571‑1398761                                                                                                 |                                                                                               aTTTAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCt                                                                                             <  1:2772549/100‑1 (MQ=255)  actAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCta                                                                                            >  1:3612003/3‑100 (MQ=255)   ttAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCtat                                                                                           >  1:5041464/1‑100 (MQ=255)    tAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCtata                                                                                          >  1:2899836/1‑100 (MQ=255)     aGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTatat                                                                                         >  1:5200175/1‑100 (MQ=255)     aGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTatat                                                                                         >  1:3772385/1‑100 (MQ=255)          tAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATaa                                                                                    <  1:4179297/100‑1 (MQ=255)          tAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATaa                                                                                    <  1:3402436/100‑1 (MQ=255)           aGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATaaa                                                                                   >  1:4941151/1‑100 (MQ=255)           aGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATaaa                                                                                   >  1:2987255/1‑100 (MQ=255)              tGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGat                                                                                >  1:3927022/1‑100 (MQ=255)                 ttattattTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATc                                                                             >  1:3999988/1‑100 (MQ=255)                    gctaaggaaaGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTg                                                                          >  1:737907/8‑100 (MQ=255)                      attTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCATGcc                                                                        <  1:3245403/100‑1 (MQ=255)                        ttAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATGTCGTGctgg                                                                      <  1:2652585/100‑4 (MQ=255)                          aaaaGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCAttt                                                                    <  1:1572774/100‑1 (MQ=255)                            aaGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGa                                                                  <  1:3291840/100‑1 (MQ=255)                                   aGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTcgc                                                           >  1:1231944/1‑100 (MQ=255)                                   aGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTcgc                                                           >  1:1057714/1‑100 (MQ=255)                                              tACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAatga                                                >  1:2922314/1‑100 (MQ=255)                                              aaCAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAatga                                                >  1:4791852/2‑100 (MQ=255)                                               tcAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAatgat                                               >  1:3687856/2‑100 (MQ=255)                                               aCAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAatgat                                               >  1:5278795/1‑100 (MQ=255)                                               aCAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAatgat                                               >  1:5667979/1‑100 (MQ=255)                                                cAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAatgatt                                              <  1:4328953/100‑2 (MQ=255)                                                 aGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGc                                             <  1:712192/100‑1 (MQ=255)                                                   tgaCATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGccc                                           >  1:1923634/3‑100 (MQ=255)                                                   cAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGccc                                           >  1:3931278/1‑100 (MQ=255)                                                     ccATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGACGCCCTa                                         >  1:3721807/2‑100 (MQ=255)                                                       aTCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGt                                       <  1:2299171/100‑1 (MQ=255)                                                         ttaTGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTaa                                     >  1:477628/3‑100 (MQ=255)                                                         cAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTaa                                     >  1:2034212/1‑100 (MQ=255)                                                         cAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTaa                                     >  1:1462661/1‑100 (MQ=255)                                                         cAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTaa                                     >  1:304732/1‑100 (MQ=255)                                                         cAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTaa                                     >  1:3009875/1‑100 (MQ=255)                                                                tggctGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGtt                              >  1:4903756/5‑100 (MQ=255)                                                                 gtatGTTAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGttt                             >  1:2600009/2‑100 (MQ=255)                                                                    gcttAAATTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTaaa                          >  1:2638398/3‑100 (MQ=255)                                                                        aaaTTAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATcac                      >  1:1800648/1‑100 (MQ=255)                                                                          acaggggcagTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACAt                    >  1:1852887/10‑100 (MQ=255)                                                                            tAGATCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATgg                  <  1:4321571/100‑1 (MQ=255)                                                                            cccggccGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATgg                  >  1:4212827/6‑100 (MQ=255)                                                                              caTCCGTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCATATGGtt                >  1:4929274/2‑100 (MQ=255)                                                                                 ccGTCTTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACg             >  1:2949804/1‑100 (MQ=255)                                                                                  agTATTGGTTATATCGTCTATATGATAAAGATATCGTGACATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGa            >  1:2866016/2‑100 (MQ=255)                                                                                   gTATTGGTTATATCGTCTATATGATAATGATCTCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAg           >  1:4120791/1‑100 (MQ=255)                                                                                   gTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGGAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAg           >  1:2931386/1‑100 (MQ=255)                                                                                   gTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAg           >  1:1159887/1‑100 (MQ=255)                                                                                   gTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAg           >  1:5355758/1‑100 (MQ=255)                                                                                   gTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAg           >  1:5015943/1‑100 (MQ=255)                                                                                   gTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAg           >  1:1515255/1‑100 (MQ=255)                                                                                   gTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAg           >  1:3245031/1‑100 (MQ=255)                                                                                   gTATTGGTTATATCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAg           >  1:2835309/1‑100 (MQ=255)                                                                                          ctataTCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAGCATCATg    >  1:3709083/2‑100 (MQ=255)                                                                                            atatCGTCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAGCATCATGGc  >  1:2367471/1‑100 (MQ=255)                                                                                                 |                                                                                               ATTTAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATTAGATCCGTATTGGTTATATCATCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCCTAGTAAACTTGTTTAAATCACATGGTTACGAGCATCATGGC  >  NC_007793/1398571‑1398761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑