Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,831	A→T	P230P (CCT→CCA)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,831	0	A	T	100.0%	93.7 / NA	32	P230P (CCT→CCA)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/28);  total (4/28)

CTTCAGCTATCACTGGTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAAAGGGTTAGCTAAGATGTATGTGATTGAACTAATACTGAAAGGTTTCCCCTTTTTAGTGACATATCCTTTGTGATTCAATGCATTGGCAATTTTACG  >  NC_007793/46740‑46926                                                                                            |                                                                                                cTTCAGCTATCACTGGTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGc                                                                                         <  1:4490326/100‑1 (MQ=255)   tCAGCTATCACTGGTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTa                                                                                       <  1:3562987/100‑1 (MQ=255)   tCAGCTATCACTGGTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTa                                                                                       <  1:1973720/100‑1 (MQ=255)   tCAGCTATCACTGGTTTATCATTCAGCCCTTTACGACGTTTTTCACGCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTa                                                                                       <  1:4100096/100‑1 (MQ=255)    cAGCTATCACTGGTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTag                                                                                      <  1:4284767/100‑2 (MQ=255)                gTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGAt                                                                          <  1:4188836/100‑1 (MQ=255)                gTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGAt                                                                          <  1:3060387/100‑1 (MQ=255)                                         tttttCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTaccc                                                 <  1:4330489/100‑1 (MQ=255)                                                     aTCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGAct                                     <  1:3527879/100‑2 (MQ=255)                                                         ttGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACatagc                                 >  1:2417541/1‑98 (MQ=255)                                                         ttGTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACatagc                                 >  1:2784622/1‑98 (MQ=255)                                                           gTATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCt                               <  1:3098719/100‑1 (MQ=255)                                                            tATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGccca                              <  1:3563574/100‑3 (MQ=255)                                                            tATTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCtt                              <  1:2232301/100‑1 (MQ=255)                                                            tATTTCGCAAATTGAATATTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGTACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCtt                              <  1:1040591/100‑1 (MQ=255)                                                             aTTTCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATAGATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCtaa                             <  1:2682796/100‑3 (MQ=255)                                                              tttCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTCCCCTTTTTAGTGACATAGCCttta                            <  1:2269635/100‑2 (MQ=255)                                                              tttCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCttag                            <  1:3057996/100‑3 (MQ=255)                                                              tttCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtg                            <  1:2783447/100‑1 (MQ=255)                                                              tttCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtg                            <  1:4552623/100‑1 (MQ=255)                                                                tCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtgtg                          <  1:3248582/100‑1 (MQ=255)                                                                tCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtgtg                          <  1:1670947/100‑1 (MQ=255)                                                                tCGCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtgtg                          <  1:1524195/100‑1 (MQ=255)                                                                  gCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTtgtgta                        <  1:365728/100‑3 (MQ=255)                                                                  gCAAATTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGAt                        <  1:3095528/100‑1 (MQ=255)                                                                    aaaTTGAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTc                      <  1:5437303/100‑1 (MQ=255)                                                                         gAATTTTGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGc                 <  1:3454481/100‑1 (MQ=255)                                                                            ttttGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCaat              <  1:1319408/100‑3 (MQ=255)                                                                            ttttGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCAtg              <  1:3002794/100‑2 (MQ=255)                                                                               tGCCGATATAGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGc           <  1:3934308/100‑1 (MQ=255)                                                                                     tataGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTAtttt     >  1:3461329/1‑100 (MQ=255)                                                                                        aGAATGGGTTAGCTAATATATATGTGATAGAACTAATACTAAAAGGTTTACCCTTTTTAGTGACATAGCCTTTGTGATTCAATGCATTGGCTATTTTACg  >  1:1110348/1‑100 (MQ=255)                                                                                            |                                                                                                CTTCAGCTATCACTGGTTTATCATTCAGCCCTTTACGACGTTTTTCACTCCAATCTTTGTATTTCGCAAATTGAATTTTGCCGATATAGAAAGGGTTAGCTAAGATGTATGTGATTGAACTAATACTGAAAGGTTTCCCCTTTTTAGTGACATATCCTTTGTGATTCAATGCATTGGCAATTTTACG  >  NC_007793/46740‑46926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑