Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,391,433	G→A	E343K (GAA→AAA)	SAUSA300_RS06855 →	anthranilate synthase subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,391,433	0	G	A	100.0%	33.1 / NA	14	E343K (GAA→AAA)	SAUSA300_RS06855	anthranilate synthase subunit I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/4);  total (10/4)

CCAAAAGAATGCAGCGAACATCGTATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTGAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGTGAAGTCACAGGTAA  >  NC_007793/1391343‑1391494                                                                                           |                                                              ccAAAAGAATGCAGCGAACATCGTATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATgg                                                      >  1:5152044/1‑100 (MQ=255)   aaaaGAATGCAGCGAACATCGTATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGtt                                                    >  1:1441860/1‑100 (MQ=255)     aaGAATGCAGCGAACATCGTATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTAt                                                  <  1:3739185/100‑1 (MQ=255)        caTGCAGCGAACATCGTATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGa                                               >  1:4692646/2‑100 (MQ=255)                      cGTATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATg                                 >  1:2585422/1‑100 (MQ=255)                       ctATGCTAGTTGATTTAGGACGTAATGATATTGATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATga                                >  1:1127532/2‑99 (MQ=255)                         aTGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATGttc                              >  1:3345419/1‑99 (MQ=255)                           gCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTaga                            >  1:3970170/1‑98 (MQ=255)                              aGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCat                         >  1:5070583/1‑100 (MQ=255)                              aGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCac                         <  1:2490524/100‑2 (MQ=255)                                       aGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGt                >  1:4985604/1‑100 (MQ=255)                                         gACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGTGa              <  1:3273256/100‑1 (MQ=255)                                               aTGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGTGAAGTcac        <  1:1587982/100‑1 (MQ=255)                                                     ttCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGTGAAGTCACAgatcg  >  1:241513/1‑96 (MQ=255)                                                                                           |                                                              CCAAAAGAATGCAGCGAACATCGTATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAAATTACTGAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGTGAAGTCACAGGTAA  >  NC_007793/1391343‑1391494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑