Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,374,405	A→C	Y80S (TAC→TCC)	SAUSA300_RS06790 →	DNA topoisomerase 4 subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,374,405	0	A	C	100.0%	184.4 / NA	68	Y80S (TAC→TCC)	SAUSA300_RS06790	DNA topoisomerase 4 subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (65/3);  total (65/3)

GCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTACTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATAATGA  >  NC_007793/1374308‑1374501                                                                                                  |                                                                                                  gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGactcct                                                                                                 >  1:1255629/1‑100 (MQ=255) gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGactcct                                                                                                 >  1:5663831/1‑100 (MQ=255)  cAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGActcctc                                                                                                <  1:3384392/100‑1 (MQ=255)   caTGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAACAGTGCGAAAAAAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCa                                                                                               >  1:4556905/2‑100 (MQ=255)        aTTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTa                                                                                          >  1:273018/1‑100 (MQ=255)        aTTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTa                                                                                          >  1:1917085/1‑100 (MQ=255)        aTTCAAGTGGTAATACACACGATAAAAATTTCCGCAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTa                                                                                          >  1:3643134/1‑100 (MQ=255)             gctGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAg                                                                                     >  1:4274808/3‑100 (MQ=255)              gTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGc                                                                                    >  1:3106102/1‑100 (MQ=255)               tGGAAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGCTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCa                                                                                   >  1:5330239/1‑100 (MQ=255)                ggTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCaa                                                                                  >  1:4775379/1‑100 (MQ=255)                ggTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCaa                                                                                  >  1:2451733/1‑100 (MQ=255)                ggTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCaa                                                                                  >  1:5525002/1‑100 (MQ=255)                ggTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCaa                                                                                  >  1:1555604/1‑100 (MQ=255)                   aaTACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATgg                                                                               >  1:3523364/1‑100 (MQ=255)                   aaTACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATgg                                                                               >  1:2516566/1‑100 (MQ=255)                         cacGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGtt                                                                         >  1:1147191/1‑100 (MQ=255)                                aaaaTTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑Ca                                                                 <  1:3695594/100‑1 (MQ=255)                                aaaaTTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑Ca                                                                 <  1:1348608/100‑1 (MQ=255)                                     ttCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACt                                                            >  1:3325417/1‑100 (MQ=255)                                     ttCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACt                                                            >  1:3665656/1‑100 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGatcgg                                                          >  1:1635708/1‑96 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGatcgg                                                          >  1:4565080/1‑96 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTgg                                                          >  1:5439607/1‑100 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTgg                                                          >  1:4310310/1‑100 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTgg                                                          >  1:5421442/1‑100 (MQ=255)                                           ccaaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGt                                                      >  1:4534953/3‑100 (MQ=255)                                           aaaaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGt                                                      >  1:2221362/1‑100 (MQ=255)                                           aaaaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGAACGGAAga                                                      >  1:3001787/1‑99 (MQ=255)                                            taaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGtt                                                     >  1:2696497/2‑100 (MQ=255)                                            gcaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGtt                                                     >  1:4934296/3‑100 (MQ=255)                                            caaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGATCGGAAgag                                                     >  1:4743465/2‑98 (MQ=255)                                            aaaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTGAGT‑CAAGATCGGAAgag                                                     >  1:4152439/1‑98 (MQ=255)                                             atttGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTa                                                    >  1:2827894/4‑100 (MQ=255)                                              aGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTAc                                                   >  1:3294120/1‑100 (MQ=255)                                                tGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTGCAAGATCGGAAgagcac                                                  >  1:1444419/1‑95 (MQ=255)                                                tGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGa                                                 >  1:5028487/1‑100 (MQ=255)                                                cgCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGa                                                 >  1:5676860/2‑100 (MQ=255)                                                agCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAACAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGa                                                 >  1:4457070/2‑100 (MQ=255)                                                 gCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGAc                                                >  1:3015174/1‑100 (MQ=255)                                                 gCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGAc                                                >  1:1681359/1‑100 (MQ=255)                                                 gCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGAc                                                >  1:1509431/1‑100 (MQ=255)                                                 gCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGAc                                                >  1:5052208/1‑100 (MQ=255)                                                 gCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGAc                                                >  1:559292/1‑100 (MQ=255)                                                 gCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACCGGAAGTTACGAc                                                >  1:2858723/1‑100 (MQ=255)                                                    aaaaCAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTTAGT‑CAAGATCGGAAgagcacacgtc                                             >  1:5011029/1‑90 (MQ=255)                                                     aaaCAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGt                                            >  1:4343496/1‑100 (MQ=255)                                                     aaaCAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGt                                            >  1:3077707/1‑100 (MQ=255)                                                              gTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGa                                   >  1:5440259/1‑100 (MQ=255)                                                                gATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGaaa                                 >  1:4263644/1‑100 (MQ=255)                                                                              atatCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTaataat                   >  1:4320412/1‑100 (MQ=255)                                                                                 gcatcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgt                >  1:43059/2‑100 (MQ=255)                                                                                  gatcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgta               >  1:2332393/2‑100 (MQ=255)                                                                                   ctcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtag              >  1:3232906/2‑100 (MQ=255)                                                                                   atcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtag              >  1:5087529/1‑100 (MQ=255)                                                                                   atcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtag              >  1:1747974/1‑100 (MQ=255)                                                                                    tcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagt             >  1:3926823/1‑100 (MQ=255)                                                                                    cccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagt             >  1:4273159/2‑100 (MQ=255)                                                                                    agcacaTGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagt             >  1:2366882/3‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagta            >  1:4689942/1‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagta            >  1:4455461/1‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagca            >  1:4006267/1‑98 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAACGCATGGTAATAATGgtagta            >  1:5138725/1‑100 (MQ=255)                                                                                       acaTGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAACGgtagagat          >  1:3611087/1‑96 (MQ=255)                                                                                        taTGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGATCGTATCg         >  1:1687443/2‑100 (MQ=255)                                                                                        caTGGAGACTCCCCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCg         >  1:3966977/1‑100 (MQ=255)                                                                                          tGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGAt       <  1:2546167/100‑1 (MQ=255)                                                                                          ccgagaCTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagatcggaa       >  1:3828011/3‑93 (MQ=255)                                                                                            aagaCTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATaa     >  1:1389365/2‑100 (MQ=255)                                                                                             agaCTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATAAt    >  1:1686305/1‑100 (MQ=255)                                                                                              gaCTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATAATg   >  1:5338796/1‑100 (MQ=255)                                                                                               actcctCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATAATGa  >  1:5294018/1‑100 (MQ=255)                                                                                               actcctCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATAATGa  >  1:1154703/1‑100 (MQ=255)                                                                                                  |                                                                                                  GCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTACTCAGTGTACGAAGCAATGGTCCGTTTAAGT‑CAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATAATGA  >  NC_007793/1374308‑1374501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑