Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,363,207	A→T	I761F (ATT→TTT)	SAUSA300_RS06750 →	nuclease SbcCD subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,363,207	0	A	T	100.0%	206.7 / NA	71	I761F (ATT→TTT)	SAUSA300_RS06750	nuclease SbcCD subunit C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (67/4);  total (67/4)

ATGACAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAAATTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGA  >  NC_007793/1363115‑1363302                                                                                             |                                                                                                atgaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCAtgaatttgaaat                                                                                          >  1:3906976/1‑100 (MQ=255)  tgaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATg                                                                                         >  1:3113682/1‑100 (MQ=255)  tgaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATg                                                                                         >  1:4223662/1‑100 (MQ=255)   gaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATgg                                                                                        >  1:451955/1‑100 (MQ=255)     cAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGaa                                                                                      >  1:5099404/1‑100 (MQ=255)           tataGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCt                                                                                <  1:5523366/100‑1 (MQ=255)                aTGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGtg                                                                           <  1:5461150/100‑2 (MQ=255)                  gAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAg                                                                         >  1:185078/1‑100 (MQ=255)                       tataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                    >  1:3736212/2‑100 (MQ=255)                       gataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                    >  1:2552476/2‑100 (MQ=255)                       cataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                    >  1:831722/2‑100 (MQ=255)                       cataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                    >  1:5016707/2‑100 (MQ=255)                       cataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                    >  1:2261208/2‑100 (MQ=255)                       aataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                    >  1:800148/1‑100 (MQ=255)                       aataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                    >  1:4996006/1‑100 (MQ=255)                       aataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                    >  1:4004335/1‑100 (MQ=255)                        ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                   >  1:3200043/1‑100 (MQ=255)                        ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                   >  1:841802/1‑100 (MQ=255)                        ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                   >  1:1259199/1‑100 (MQ=255)                        ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                   >  1:2573209/1‑100 (MQ=255)                        ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCCAGGtagatcggaa                                                                   >  1:578074/1‑91 (MQ=255)                         taGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAtt                                                                  >  1:614449/1‑100 (MQ=255)                         taGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAtt                                                                  >  1:3584291/1‑100 (MQ=255)                          aGCCTGGAGGGGCGAGCAAGAGGCATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                 >  1:640047/1‑100 (MQ=255)                          aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                 >  1:3221656/1‑100 (MQ=255)                          aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                 >  1:4514749/1‑100 (MQ=255)                          aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                 >  1:3733904/1‑100 (MQ=255)                          aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                 >  1:5533393/1‑100 (MQ=255)                          aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                 >  1:1835774/1‑100 (MQ=255)                          aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                 >  1:99477/1‑100 (MQ=255)                          aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                 >  1:1059087/1‑100 (MQ=255)                            ccTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAAc                                                               >  1:3644470/1‑100 (MQ=255)                            ccTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTCATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAAc                                                               >  1:1478912/1‑100 (MQ=255)                             ttgtgggggCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACt                                                              >  1:1280903/6‑100 (MQ=255)                               ggAGGGGCGTGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTaa                                                            >  1:2477052/1‑100 (MQ=255)                               ggAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTaa                                                            >  1:2258408/1‑100 (MQ=255)                               ggAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTaa                                                            >  1:781370/1‑100 (MQ=255)                                 tggggCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAgg                                                          <  1:1141685/99‑1 (MQ=255)                                 aGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAgg                                                          <  1:993142/100‑1 (MQ=255)                                 aGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAgg                                                          >  1:5209287/1‑100 (MQ=255)                                           cagagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGtt                                                >  1:4200282/2‑100 (MQ=255)                                                   ttAGCGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGAct                                        >  1:1673500/1‑100 (MQ=255)                                                   ctAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGAct                                        >  1:2603182/2‑100 (MQ=255)                                                     agagCAGAAAAGGGATATTTATAAAAAATGTTACGATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAATTAAGGATAAAGAGCTTTTGGActct                                      >  1:3718134/1‑100 (MQ=255)                                                     agagCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActct                                      >  1:3885166/1‑100 (MQ=255)                                                     agagCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActct                                      >  1:1678062/1‑100 (MQ=255)                                                      tagCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTg                                     >  1:1951591/2‑100 (MQ=255)                                                       agCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGa                                    >  1:2611428/1‑100 (MQ=255)                                                        gCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                   >  1:3662093/1‑100 (MQ=255)                                                        acAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                   >  1:2454995/2‑100 (MQ=255)                                                         gcgAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATa                                  >  1:3689651/3‑100 (MQ=255)                                                         ctgAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATa                                  >  1:587746/3‑100 (MQ=255)                                                          aGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATaa                                 >  1:8784/1‑100 (MQ=255)                                                           gAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATaaa                                >  1:1743520/1‑100 (MQ=255)                                                           gAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATaaa                                >  1:2182942/1‑100 (MQ=255)                                                            caaaGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAAt                               >  1:1166213/2‑100 (MQ=255)                                                               aGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTaa                            >  1:1272190/1‑100 (MQ=255)                                                               aGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTaa                            >  1:1843618/1‑100 (MQ=255)                                                               aGGGATACTTATAAAAAACGTTATCATGAATTTGAAAGGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTaa                            >  1:4664943/1‑100 (MQ=255)                                                                ggggTACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATCaaa                           >  1:4670979/1‑100 (MQ=255)                                                                     aCTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATg                      >  1:3638433/1‑100 (MQ=255)                                                                        tataAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAat                   >  1:323738/1‑100 (MQ=255)                                                                                 cGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATACATTAAAAGATGTATATGAGCTaa          >  1:1672907/1‑100 (MQ=255)                                                                                    tATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATTATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaa       >  1:4247720/1‑100 (MQ=255)                                                                                    tATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaa       >  1:5686251/1‑100 (MQ=255)                                                                                    tATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaa       >  1:4316210/1‑100 (MQ=255)                                                                                     ctCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaaa      >  1:5600660/2‑100 (MQ=255)                                                                                      ccATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTcaaaaaa     >  1:1720173/2‑100 (MQ=255)                                                                                      acATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaata     >  1:3239499/2‑98 (MQ=255)                                                                                       cATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaaatg    >  1:3260165/1‑98 (MQ=255)                                                                                       cATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaaacg    >  1:2955162/1‑98 (MQ=255)                                                                                       cATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAg    >  1:3716775/1‑100 (MQ=255)                                                                                         tgaatttgaaatGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaagatcggaa  >  1:2297629/1‑92 (MQ=255)                                                                                             |                                                                                                ATGACAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAAATTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGA  >  NC_007793/1363115‑1363302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑