Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,338,438	T→A	F52I (TTT→ATT)	SAUSA300_RS06615 →	DNA‑binding response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,338,438	0	T	A	100.0%	145.9 / NA	53	F52I (TTT→ATT)	SAUSA300_RS06615	DNA‑binding response regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (52/1);  total (52/1)

TTAATTAAACTACATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTTTTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCATTTGAATATTAAAGTGATTATTGTAA  >  NC_007793/1338342‑1338528                                                                                                 |                                                                                           ttaattaaACTACATGGTGATTTTGAAATTCTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGttattt                                                                                         >  1:4373153/1‑100 (MQ=255)          cTACATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAg                                                                                >  1:4086198/1‑100 (MQ=255)            aCATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGaa                                                                              >  1:3444407/1‑100 (MQ=255)            aCATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGaa                                                                              >  1:5594000/1‑100 (MQ=255)            aCATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGaa                                                                              >  1:3551883/1‑100 (MQ=255)            aCATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGaa                                                                              >  1:5117118/1‑100 (MQ=255)            aCATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGaa                                                                              >  1:1898500/1‑100 (MQ=255)            aCATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGaa                                                                              >  1:2361951/1‑100 (MQ=255)            aCATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGaa                                                                              >  1:4700040/1‑100 (MQ=255)                ggTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGc                                                                          >  1:2725635/1‑100 (MQ=255)                 gTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGcc                                                                         <  1:2691680/100‑1 (MQ=255)                       ttGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAGACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCAt                                                                   >  1:1234742/1‑100 (MQ=255)                               ttAGCAGATACTGATAATGGTCTCGATGCAATGATACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGAc                                                           >  1:1514584/1‑100 (MQ=255)                                tAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCaaatcggaaga                                                          >  1:4707882/1‑90 (MQ=255)                                tAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACt                                                          >  1:4387696/1‑100 (MQ=255)                                tAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACt                                                          >  1:5197651/1‑100 (MQ=255)                                tAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACt                                                          >  1:3518867/1‑100 (MQ=255)                                 aGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACtt                                                         >  1:1037733/1‑100 (MQ=255)                                   cAGATACTGATAATGGCCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGa                                                       >  1:297114/1‑100 (MQ=255)                                    aGATACTGATAATGGTCTCGATGCAGTGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGaa                                                      >  1:4801619/1‑100 (MQ=255)                                    aGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGaa                                                      >  1:79210/1‑100 (MQ=255)                                        aCTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGttt                                                  >  1:2246709/1‑100 (MQ=255)                                        aCTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGttt                                                  >  1:4467427/1‑100 (MQ=255)                                        aCTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGttt                                                  >  1:4451994/1‑100 (MQ=255)                                         cTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGtttt                                                 >  1:3665177/1‑100 (MQ=255)                                           gATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAGGTTTTAg                                               >  1:3318290/1‑100 (MQ=255)                                           gATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTaa                                               >  1:1297520/1‑99 (MQ=255)                                           gATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAg                                               >  1:3371172/1‑100 (MQ=255)                                           gATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAg                                               >  1:2036691/1‑100 (MQ=255)                                           gATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAg                                               >  1:2328804/1‑100 (MQ=255)                                           gATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAg                                               >  1:4113604/1‑100 (MQ=255)                                           gATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAg                                               >  1:3537330/1‑100 (MQ=255)                                           gATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAg                                               >  1:2528397/1‑100 (MQ=255)                                              aaTGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCgg                                            >  1:2382983/1‑100 (MQ=255)                                              aaTGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGATTTAGCgg                                            >  1:4771273/1‑100 (MQ=255)                                                 ggTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGaaa                                         >  1:29453/1‑100 (MQ=255)                                                 ggTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCAGaag                                         >  1:3634699/1‑99 (MQ=255)                                                      cGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGtaagatcg                                    >  1:3398328/1‑95 (MQ=255)                                                      cGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGaagatcgg                                    >  1:3516666/1‑94 (MQ=255)                                                      cGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGa                                    >  1:2559161/1‑100 (MQ=255)                                                      cGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGa                                    >  1:4033973/1‑100 (MQ=255)                                                      cGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGa                                    >  1:1629715/1‑100 (MQ=255)                                                      cGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGATTTAGCGGaagatcgg                                    >  1:4759952/1‑94 (MQ=255)                                                         tGCAATGAAACTTATTGAAGAATATAATCCTAACGCTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGaaaa                                 >  1:4814427/1‑100 (MQ=255)                                                          gCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGTTATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGaaaaa                                >  1:1395108/1‑100 (MQ=255)                                                          gCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGaagatcggaaga                                >  1:5484613/1‑90 (MQ=255)                                                          gCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGaaaaa                                >  1:3732286/1‑100 (MQ=255)                                                               gAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCAt                           >  1:2328363/1‑100 (MQ=255)                                                               gAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCAt                           >  1:2129739/1‑100 (MQ=255)                                                               gAAACTTATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCAt                           >  1:5363866/1‑100 (MQ=255)                                                                 tccgtATTGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCAttt                         >  1:3556640/5‑100 (MQ=255)                                                                        tGAAGAATATAATCCTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCATTTGAATAtt                  >  1:3888510/1‑100 (MQ=255)                                                                                     ccTAACGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCATTTGAATATTAAAGTGATTATTg     >  1:1615481/1‑100 (MQ=255)                                                                                        aaCGTTGTTATTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCATTTGAATATTAAAGTGATTATTGTaa  >  1:4282207/1‑100 (MQ=255)                                                                                                 |                                                                                           TTAATTAAACTACATGGTGATTTTGAAATTTTAGCAGATACTGATAATGGTCTCGATGCAATGAAACTTATTGAAGAATATAATCCTAACGTTGTTTTTTTAGATATAGAAATGCCAGGCATGACTGGACTTGAAGTTTTAGCGGAAATTAGAAAAAAGCATTTGAATATTAAAGTGATTATTGTAA  >  NC_007793/1338342‑1338528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑