Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,320,950	C→T	T14M (ACG→ATG)	SAUSA300_RS06475 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,320,950	0	C	T	100.0%	43.5 / NA	16	T14M (ACG→ATG)	SAUSA300_RS06475	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/0);  total (16/0)

ATCGTATAAAAGGATTTAAAAAATAATAAAAGGACGAAATTCAAATGAAAGATATAAGTAAGATAGTAGCTGACGTCGAATCAACGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTCAAAGCAACCGAAAGTG  >  NC_007793/1320866‑1321045                                                                                     |                                                                                                aTCGTATAAAAGGATTTAAAAAATAATAAAAGGACGAAATTCAAATGAAAGATATAAGTAAGATAGTAGCTGACGTCGAATCAATGTTAGCACCATAttt                                                                                  >  1:1981346/1‑100 (MQ=255)                             aaaGGACGAAATTCAAATGAAAGATATAAGGAAGATAGTAGCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATAtt                                                      >  1:1663015/1‑100 (MQ=255)                              aaGGACGAAATTCAAATGAAAGATATAAGTAAGATAGTAGCTGACGCCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTa                                                     >  1:3310751/1‑100 (MQ=255)                                                        aagtaagATAGTAGCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCAttt                           >  1:263124/1‑100 (MQ=255)                                                        aagtaagATAGTAGCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCAttt                           >  1:4177550/1‑100 (MQ=255)                                                           taagATAGTAGCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTtcat                        >  1:5603522/1‑100 (MQ=255)                                                            aagATAGTAGCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTtcatc                       >  1:4335949/1‑100 (MQ=255)                                                              gATAGTAGCTGACGTCGAATCAATGTTAGCACCATATTTTAATGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTcatcat                     >  1:4823113/1‑100 (MQ=255)                                                                tagtagCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATGTATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGt                   >  1:1409949/1‑100 (MQ=255)                                                                 agtagCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTc                  >  1:1105928/1‑100 (MQ=255)                                                                 agtagCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTc                  >  1:4846377/1‑100 (MQ=255)                                                                    agCTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTCaaa               >  1:5278910/1‑100 (MQ=255)                                                                      cTGACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTCAAAGc             >  1:344841/1‑100 (MQ=255)                                                                        gACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTCAAAGCaa           >  1:3746058/1‑100 (MQ=255)                                                                        gACGTCGAATCAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTCAAAGCaa           >  1:4558648/1‑100 (MQ=255)                                                                                 acAATGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTCAAAGCAACCGAAAGTg  >  1:2880689/2‑100 (MQ=255)                                                                                     |                                                                                                ATCGTATAAAAGGATTTAAAAAATAATAAAAGGACGAAATTCAAATGAAAGATATAAGTAAGATAGTAGCTGACGTCGAATCAACGTTAGCACCATATTTTAAAGAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTCAAAGCAACCGAAAGTG  >  NC_007793/1320866‑1321045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑