Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,295,497	A→G	A207A (GCA→GCG)	SAUSA300_RS06365 →	competence protein CinA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,295,497	0	A	G	100.0%	165.6 / NA	62	A207A (GCA→GCG)	SAUSA300_RS06365	competence protein CinA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (61/1);  total (61/1)

TCTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCACCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAACAAGAA  >  NC_007793/1295405‑1295593                                                                                             |                                                                                                 gcTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTgg                                                                                           >  1:377676/2‑100 (MQ=255)  cTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGc                                                                                          >  1:1403573/1‑100 (MQ=255)  atGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGc                                                                                          >  1:238671/2‑100 (MQ=255)    gAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTtggtgg                                                                                        >  1:3939572/1‑100 (MQ=255)    gAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCgg                                                                                        >  1:2665106/1‑100 (MQ=255)    gAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCgg                                                                                        >  1:4707979/1‑100 (MQ=255)              gATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGagatcggaa                                                                              >  1:2723095/1‑92 (MQ=255)                 ttGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                           >  1:73135/1‑100 (MQ=255)                 ttGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                           >  1:3601444/1‑100 (MQ=255)                 ttGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGt                                                                           >  1:219398/1‑100 (MQ=255)                  tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                          >  1:5394549/1‑100 (MQ=255)                  tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                          >  1:4521205/1‑100 (MQ=255)                  tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                          >  1:5653733/1‑100 (MQ=255)                  atggggAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                          >  1:4622330/4‑100 (MQ=255)                   gCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGtat                                                                         >  1:5545718/1‑100 (MQ=255)                        taTAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATAtt                                                                    >  1:1487399/2‑100 (MQ=255)                         aTAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTa                                                                   >  1:3255735/1‑100 (MQ=255)                           aGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGa                                                                 >  1:3246382/1‑100 (MQ=255)                           aGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGa                                                                 >  1:5403178/1‑100 (MQ=255)                           aGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGa                                                                 >  1:1618972/1‑100 (MQ=255)                           aGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGa                                                                 >  1:4125955/1‑100 (MQ=255)                               gAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGa                                                             >  1:1912457/1‑100 (MQ=255)                                     aaaGTAGAAACAATATTAGTAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                       >  1:1166979/1‑100 (MQ=255)                                     aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                       >  1:3379254/1‑100 (MQ=255)                                     aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                       >  1:3497319/1‑100 (MQ=255)                                     aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                       >  1:4666788/1‑100 (MQ=255)                                                               tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGatcggaagagc                             >  1:473815/1‑90 (MQ=255)                                                               tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                             >  1:5622247/1‑100 (MQ=255)                                                               tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                             >  1:4304953/1‑100 (MQ=255)                                                                aTCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAAt                            >  1:1608352/1‑100 (MQ=255)                                                                      aaaCAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGa                      >  1:3667439/1‑100 (MQ=255)                                                                        aCAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGAtt                    >  1:352546/1‑100 (MQ=255)                                                                            aCTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAAc                >  1:1974113/1‑100 (MQ=255)                                                                              tgaTCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCt              >  1:1866948/3‑100 (MQ=255)                                                                               gtgccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTg             >  1:2729268/4‑100 (MQ=255)                                                                               aaTCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTg             >  1:1342210/1‑100 (MQ=255)                                                                               aaTCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTg             >  1:4537726/1‑100 (MQ=255)                                                                                aTCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGt            >  1:1478908/1‑100 (MQ=255)                                                                                 cccTGCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGtt           >  1:3553422/2‑100 (MQ=255)                                                                                  ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa          >  1:4829647/1‑100 (MQ=255)                                                                                  ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa          >  1:5270654/1‑100 (MQ=255)                                                                                  ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa          >  1:3594357/1‑100 (MQ=255)                                                                                  ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa          >  1:3266315/1‑100 (MQ=255)                                                                                  ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa          >  1:5630742/1‑100 (MQ=255)                                                                                  ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa          >  1:2290528/1‑100 (MQ=255)                                                                                  cagatgATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa          >  1:1046777/6‑100 (MQ=255)                                                                                   gtACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTaa         >  1:5576108/2‑100 (MQ=255)                                                                                   cTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTaa         >  1:4684018/1‑100 (MQ=255)                                                                                    tACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTaaa        >  1:563666/1‑100 (MQ=255)                                                                                    ctcGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTaaa        >  1:2077875/3‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTTTTAAAc       >  1:877394/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:5250107/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:4733152/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:4168282/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:308342/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:2357048/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:2110236/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:1617879/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:72259/1‑100 (MQ=255)                                                                                     aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc       >  1:1381917/1‑100 (MQ=255)                                                                                        aTTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAACAAg    <  1:4703142/100‑1 (MQ=255)                                                                                          tGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAACAAGaa  >  1:1144983/1‑100 (MQ=255)                                                                                             |                                                                                                 TCTGAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCACCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAACAAGAA  >  NC_007793/1295405‑1295593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑