Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,269,542	A→T	I18I (ATA→ATT)	polC →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,269,542	0	A	T	100.0%	177.0 / NA	60	I18I (ATA→ATT)	polC	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (59/1);  total (59/1)

GTTCGAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATATCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAA  >  NC_007793/1269445‑1269623                                                                                                  |                                                                                  gTTCGAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATttc                                                                                 >  1:3724436/1‑100 (MQ=255) attCGAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATttc                                                                                 >  1:5780788/2‑100 (MQ=255)     gAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATttcaaat                                                                             >  1:4880861/1‑100 (MQ=255)      aaCACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATttcaaatc                                                                            >  1:5634199/1‑100 (MQ=255)         acGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAAt                                                                         >  1:637203/1‑100 (MQ=255)             aaaaGTAATTTCTATTTAAGGGGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGa                                                                     >  1:250555/1‑100 (MQ=255)                     tttCTATTTAAGGTGGTAATTGTCTTGTCAATGACAGAGCAACAAAAATTTAAAGTGCTTTCTGATCAAATTAAAATTTCAAATCAATTAGATGTTGaaa                                                             >  1:906002/1‑100 (MQ=255)                        cTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAAttt                                                          >  1:542437/1‑100 (MQ=255)                          aTTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTa                                                        >  1:5207715/1‑100 (MQ=255)                          aTTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTa                                                        >  1:5516788/1‑100 (MQ=255)                          aTTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTa                                                        >  1:4636229/1‑100 (MQ=255)                           cttAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTaa                                                       >  1:2164438/2‑100 (MQ=255)                            ttAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTaaa                                                      >  1:353806/1‑100 (MQ=255)                             tAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAAt                                                     >  1:489892/1‑100 (MQ=255)                              aaGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAAtt                                                    >  1:418731/1‑100 (MQ=255)                                 ttggtAATTGTCTTGGCAATGACAGAGTAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAg                                                 >  1:717720/2‑100 (MQ=255)                                 gtggtAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAg                                                 >  1:3415360/1‑100 (MQ=255)                                            cTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                      >  1:4715231/1‑100 (MQ=255)                                            cTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                      >  1:3182598/1‑100 (MQ=255)                                            cTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                      >  1:4800364/1‑100 (MQ=255)                                             ttGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                     >  1:4177895/1‑100 (MQ=255)                                             ttGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                     >  1:296142/1‑100 (MQ=255)                                             ttGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                     >  1:3971837/1‑100 (MQ=255)                                             ctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                     >  1:1068399/2‑100 (MQ=255)                                             ctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacaa                                     >  1:5670578/2‑99 (MQ=255)                                             ctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTCCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGAGAACTGacac                                     >  1:2588566/2‑100 (MQ=255)                                              tGGCAGTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:654308/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAACAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:2792793/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:369713/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:1487402/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:1499727/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:1779293/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:5373307/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:4289850/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:2783407/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:4184714/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:701958/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:4142944/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:4093304/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:3029746/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:667482/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:3498151/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:3575136/1‑100 (MQ=255)                                              tGGCAATGACAGAGCAACAAAAATTTAAAGCGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                    >  1:687766/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGagatcggaaga                                   >  1:2712284/1‑90 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:1144364/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:5024034/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:4219646/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:3986376/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:3724517/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:3716561/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:3356705/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:2865060/1‑100 (MQ=255)                                               ggCAATGACAGAGCAACAAAAATTTAAAGCGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                   >  1:3972449/1‑100 (MQ=255)                                                 cAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGtat                                 <  1:479605/100‑1 (MQ=255)                                                   aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                               >  1:529539/1‑100 (MQ=255)                                                   aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                               >  1:2185708/1‑100 (MQ=255)                                                   aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                               >  1:663213/1‑100 (MQ=255)                                                   aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                               >  1:2830568/1‑100 (MQ=255)                                                       cAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGt                           >  1:4096668/1‑100 (MQ=255)                                                          tgCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTc                        >  1:3853044/2‑100 (MQ=255)                                                                            tgcttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATg      >  1:5285430/1‑100 (MQ=255)                                                                             gcttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATgg     >  1:5356982/1‑100 (MQ=255)                                                                                tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGaa  >  1:1287982/1‑100 (MQ=255)                                                                                                  |                                                                                  GTTCGAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATATCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAA  >  NC_007793/1269445‑1269623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑