Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,657	G→A	V288V (GTC→GTT)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,657	0	G	A	100.0%	123.3 / NA	43	V288V (GTC→GTT)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/34);  total (9/34)

GTCTTTAAGAGTATTCGTGGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAGTGAATAATGCCTGTAAGCAGATTCGTTCCTTTGCCATGGACTTGGGGTTTTTGACTGACTTGTTTCTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTACCTTCAGCTATCA  >  NC_007793/46561‑46751                                                                                                 |                                                                                               atCTTTAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCTACATTGGGGACAATGGATAATGACTGTAAGCAGATTCTTTCCTTTACCATGAACt                                                                                             <  1:222153/99‑1 (MQ=255)   cTTTAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTg                                                                                           <  1:5708575/100‑1 (MQ=255)   cTTTAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTg                                                                                           <  1:539376/100‑1 (MQ=255)    tttAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTgg                                                                                          <  1:1992922/100‑1 (MQ=255)     ttAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTggg                                                                                         <  1:1353182/100‑1 (MQ=255)     ttAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTggg                                                                                         <  1:3387143/100‑1 (MQ=255)     ttAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTggg                                                                                         >  1:2815035/1‑100 (MQ=255)     ttAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTggc                                                                                         <  1:3389355/100‑2 (MQ=255)     ttAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTgat                                                                                         <  1:777491/100‑3 (MQ=255)      tAAGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTgggg                                                                                        <  1:1500225/100‑1 (MQ=255)       aaGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTggggg                                                                                       <  1:2039236/100‑2 (MQ=255)       aaGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGt                                                                                       <  1:971613/100‑1 (MQ=255)       aaGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGt                                                                                       <  1:885988/100‑1 (MQ=255)       aaGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGt                                                                                       >  1:4959878/1‑100 (MQ=255)       aaGTGTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGACTGTAAGCAGATTCGTTCCTTTACCATGAACTTggggg                                                                                       <  1:5685764/100‑2 (MQ=255)        agtgTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGtt                                                                                      <  1:900392/97‑1 (MQ=255)        agtgTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGtt                                                                                      <  1:2708269/97‑1 (MQ=255)         gtgTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGttt                                                                                     <  1:3717183/98‑1 (MQ=255)         gtgTATTCGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCATTTACCATGAACTTGGGGtaa                                                                                     <  1:746529/98‑3 (MQ=255)                cGTTGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTtgactg                                                                              <  1:2384093/100‑1 (MQ=255)                      aTTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTgg                                                                        <  1:2002620/100‑2 (MQ=255)                              ctgcCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACg                                                                >  1:3294330/1‑100 (MQ=255)                               tgcCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGc                                                               <  1:156571/100‑1 (MQ=255)                                gcCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCa                                                              >  1:5728413/1‑100 (MQ=255)                                gcCATAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCa                                                              >  1:446024/1‑100 (MQ=255)                                 ccgtAGGTGCGCCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCAt                                                             >  1:2791684/4‑100 (MQ=255)                                         gcgcCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTTCCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACta                                                     <  1:404656/100‑2 (MQ=255)                                         gcgcCACATTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACtt                                                     >  1:2553641/1‑100 (MQ=255)                                              acaTTGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATcc                                                <  1:3217511/100‑1 (MQ=255)                                                 ttGGGGACAATTGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGAGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCAt                                             <  1:3718240/100‑1 (MQ=255)                                                 ttGGGGACAATGGATAATGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCAt                                             <  1:3788460/100‑1 (MQ=255)                                                        caatggataatGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCtt                                      <  1:3758372/94‑1 (MQ=255)                                                         aatggataatGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTg                                     <  1:1912494/95‑1 (MQ=255)                                                               caatGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATtaat                               >  1:4542211/2‑100 (MQ=255)                                                               agatGCCTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATtaat                               >  1:829127/3‑100 (MQ=255)                                                                    ccTGTAAGCAGATTCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATgg                          <  1:3902717/100‑1 (MQ=255)                                                                                ttCGTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTAc              <  1:1781260/100‑1 (MQ=255)                                                                                   gTTCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTACCtt           <  1:3947526/100‑1 (MQ=255)                                                                                    ttCCTTTACCATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTAccctg          <  1:1625192/100‑4 (MQ=255)                                                                                          taccATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTACCTTCAGCTAt    <  1:2171422/98‑1 (MQ=255)                                                                                           accATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTACCTTCAGCTAtg   <  1:359361/99‑2 (MQ=255)                                                                                            ccATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTACCTTCAGCTacta  <  1:5071557/100‑4 (MQ=255)                                                                                            ccATGAACTTGGGGTTTTTGACTGACTTGTTTTTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTACCTTCAGCTATCa  <  1:1127563/100‑1 (MQ=255)                                                                                                 |                                                                                               GTCTTTAAGAGTATTCGTGGTATTGCTTGCTGCCATAGGTGCGCCACATTGGGGACAGTGAATAATGCCTGTAAGCAGATTCGTTCCTTTGCCATGGACTTGGGGTTTTTGACTGACTTGTTTCTTACGCATTTGTACTTTATCCCATAAATCTTGATTAATAATGGGGGAATGCTTACCTTCAGCTATCA  >  NC_007793/46561‑46751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑